Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ITM2B CRISPR/Cas9 KO Plasmid (m): sc-421189
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ITM2B CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ITM2B genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ITM2B Antibody (C-8): sc-374362
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ITM2B CRISPR/Cas9 KO Plasmid (m) | sc-421189 | 20 µg | $397.00 |
Overview
Itm2b encodes the integral membrane protein ITM2B (also known as BRI2), a type II transmembrane protein implicated in membrane trafficking and proteolytic processing within the secretory and endolysosomal systems. ITM2B participates in neuronal homeostasis and has been linked to regulation of amyloidogenic peptide production through interactions that influence APP processing. In mouse, Itm2b expression is enriched in the nervous system and is used to study pathways governing synaptic maintenance, protein quality control, and lysosomal function. Altered ITM2B biology is associated with neurodegeneration-related phenotypes and provides a mechanistic entry point for investigating proteostasis imbalance and age-related neuropathology.
ITM2B CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Itm2b gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Itm2b together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Itm2b open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ITM2B protein expression.
This CRISPR knockout system enables efficient generation of Itm2b-deficient cell models for investigation of ITM2B signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Itm2b exon(s) critical for ITM2B function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Itm2b genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ITM2B CRISPR/Cas9 KO Plasmid (m) and ITM2B CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Itm2b locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ITM2B HDR Plasmid (m) and ITM2B HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Itm2b homology arms to support homology-directed repair at defined Itm2b target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.