Date published: 2025-9-9

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IL-6 Anticorpo (10E5): sc-57315

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Fichas de dados
  • IL-6 Anticorpo 10E5é um anticorpo monoclonal produzido em camundongo IgG2b κ, citado em 166 publicações, fornecido em 100 µg/ml
  • activada contra IL-6 de comprimento total de origem rat
  • recomendado para a detecção de IL-6 de mouse e rat origem em
  • Entre em contato conosco e receba GRATUITAMENTE um amostra de 10 µg de IL-6 (10E5): sc-57315.
  • m-IgG Fc BP-HRP e 2b BP-HRP">m-IgG2b BP-HRP são os reagentes de deteção secundários preferidos para IL-6 Antibody (10E5) para aplicações WB. Estes reagentes são agora oferecidos em conjuntos com IL-6 Antibody (10E5)(ver informações de encomenda abaixo).

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VEJA TAMBÉM

O anticorpo IL-6 (10E5) é um anticorpo IL-6 monoclonal de camundongo IgG2b κ (também designado anticorpo IL-6 ou anticorpo Interleucina 6) que detecta IL-6 de origem de camundongo e rato por WB, IP, FCM e ELISA. O anticorpo IL-6 (10E5) está disponível na forma de anticorpo anti-IL-6 não conjugado. A interleucina-6, ou IL-6, é uma proteína multifuncional, com 212 aminoácidos de comprimento, que desempenha papéis essenciais na defesa do hospedeiro, na resposta imune e na hematopoiese. A IL-6 é expressa constitutivamente pelas células de Langerhans epidérmicas e sua expressão é induzida em queratinócitos estimulados. A IL-6, a IL-1β e o TNFα atuam como pirogênios endógenos, regulando a resposta febril à invasão bacteriana. O receptor de IL-6 é um complexo trimérico composto por uma cadeia α específica de IL-6 e um homodímero da glicoproteína gp130 comum aos receptores de IL-6, IL-11, CNTF, OSM e LIF. A estimulação com IL-6 leva à homodimerização da gp130 e à ativação das quinases associadas JAK1 e JAK2. Uma vez ativadas, JAK1 e JAK2 fosforilam Stat3, causando sua translocação nuclear e transcrição de genes responsivos a Stat3. Também foi mostrado que a IL-6 ativa a via Ras/MAP quinase, que regula a transcrição do NFIL6.

Para uso em exclusivo em pesquisa. Não se destina a uso em diagnostico e tratamento.

Informacoes sobre ordens

Nome do ProdutoNumero de CatalogoUNIDPrecoQdeFAVORITOS

IL-6 Anticorpo (10E5)

sc-57315
100 µg/ml
$316.00

Pacote do IL-6 (10E5): m-IgG Fc BP-HRP

sc-539053
100 µg Ab; 10 µg BP
$354.00

Pacote do IL-6 (10E5): m-IgG2b BP-HRP

sc-549728
100 µg Ab; 10 µg BP
$354.00

How can I diluete for IL-6 product with BSA or milk powder? Which do you suggest dilution rate For WB?

Asked by: ayse
Thank you for your question. We recommend using UltraCruz® Blocking Reagent: sc-516214, which is the reagent we employ when testing our antibodies.
Answered by: Tech Support Europe
Date published: 2025-01-28

how i purchase il-6 antibody in India from Jaipur (Rajasthan)

Asked by: kavita2021
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-05-26

Can we use this IL-6 (10E5) antibody (sc-57315) for IHC?

Asked by: Abhi
Thank you for your question. IL-6 Antibody (10E5): sc-57315 is only recommended for detection of IL-6 of mouse and rat origin by WB, IP, FCM and ELISA. We do not recommend it for use in IHC or IHC(P) applications.
Answered by: Tech Support Europe
Date published: 2022-07-14

What is the molecular weight of IL-6 ?

Asked by: AbPolly
Thank you for your question. IL-6 has an expected molecular weight of approximately 21 kDa. If you have any questions, please contact our Technical Service Department and we would be happy to assist you.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 3 out of 5 by from WB on rat hippocampusThe sc-57315 antibody was tested (1: 200,ON 4°) on samples extracted from rat hippocampus using a 12% acrylamide gel. In figure (from left to right): 30ug, 60ug, 80ug and 100ug of extract. The signal was slightly non-specific, but a more intense band was observed around 21kDa, as suggested by the datasheet. [Marker (from the bottom): 11,17,22,25,32 kDa]
Date published: 2022-06-23
Rated 3 out of 5 by from It doesn't work in WB of secretome of C2C12We have used this antibody to detect Il6 on conditioned medium of C2C12 cells at 1:1000 diluition and it doesn't work. It could be that the amount of IL6 produced by these cells is too low
Date published: 2018-10-05
Rated 5 out of 5 by from Works well in rat frozen liver sectionsRat liver frozen sections were subjected to immunofluorescence using IL-6 (10E5) antibody. Antigen retrieval methods were optimized between without antigen retrieval, heat-induced citrate buffer, and trypsin-induced antigen retrieval. Positive signals in portal tracts were clear without any antigen retrieval.
Date published: 2018-03-14
Rated 5 out of 5 by from Work very wellHave been using this antibody for Western blot detecting IL-6 in mouse tissue and C2C12 samples. Always got clear, specific band. would like to recommend it.
Date published: 2018-03-11
Rated 4 out of 5 by from Good!I got a small sample and tried it for WB in a dilution of 1:300. The results were great.
Date published: 2018-03-02
Rated 5 out of 5 by from Skeletal muscle immunostainingBright staining of nuclei in frozen skeletal muscle samples.
Date published: 2017-11-22
Rated 4 out of 5 by from Good antibodyformalin fixed rat brain , IF staining, 1:100 dilution. got good result
Date published: 2017-10-24
Rated 3 out of 5 by from 总体效果一般该抗体不能与人体组织反应,是一个重要的缺陷。在使用过程中,在动物组织中检测该蛋白的表达改变,需要适当增加上样量,提高1抗的浓度。
Date published: 2017-09-28
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IL-6 Anticorpo (10E5) is rated 4.2 out of 5 by 27.
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