HPV16 E7 Antibody (716-325): sc-51951

HPV16 E7 Antibody (716-325) is a mouse monoclonal IgG2a kappa light chain antibody that detects the E7 protein of HPV-16 origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-HPV16 E7 antibody (716-325) is available in a non-conjugated form, specifically designed for researchers studying the role of the E7 protein in oncogenesis. The E7 protein is a small zinc-binding phosphoprotein that plays a critical role in the regulation of the cell cycle by interacting with the retinoblastoma tumor suppressor protein (pRb). This interaction leads to the disruption of pRb′s function, promoting uncontrolled cell proliferation, a hallmark of cancer. The E7 protein contains a conserved LXCXE motif that is essential for binding to pRb, and high-risk HPV E7 proteins, such as HPV16 E7, exhibit a significantly higher affinity for pRb compared to their low-risk counterparts. This increased binding affinity facilitates the degradation of Rb family proteins, further enhancing the oncogenic potential of HPV16. The presence of the casein kinase II phosphorylation site within the E7 protein also suggests that post-translational modifications may play a role in activity and stability, making E7 a vital target for therapeutic intervention in HPV-related malignancies. Given the strong association of HPV16 and HPV18 with cervical, vaginal, and vulvar cancers, HPV16 E7 monoclonal antibody (716-325) is an invaluable tool for researchers investigating the molecular mechanisms of HPV-induced tumorigenesis.

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HPV16 E7 Antibody (716-325) References:

1. RNA interference against HPV16 E7 oncogene leads to viral E6 and E7 suppression in cervical cancer cells and apoptosis via upregulation of Rb and p53.  |  Sima, N., et al. 2008. Apoptosis. 13: 273-81. PMID: 18060502
2. Ii-Key/HPV16 E7 hybrid peptide immunotherapy for HPV16+ cancers.  |  Xu, M., et al. 2009. Vaccine. 27: 4641-7. PMID: 19520206
3. The high-risk HPV16 E7 oncoprotein mediates interaction between the transcriptional coactivator CBP and the retinoblastoma protein pRb.  |  Jansma, AL., et al. 2014. J Mol Biol. 426: 4030-4048. PMID: 25451029
4. HPV16-E7-Specific Activated CD8 T Cells in E7 Transgenic Skin and Skin Grafts.  |  Jazayeri, SD., et al. 2017. Front Immunol. 8: 524. PMID: 28523003
5. Enhancing antitumor immunogenicity of HPV16-E7 DNA vaccine by fusing DNA encoding E7-antigenic peptide to DNA encoding capsid protein L1 of Bovine papillomavirus.  |  Yang, A., et al. 2017. Cell Biosci. 7: 46. PMID: 28852471
6. Murine HPV16 E7-expressing transgenic skin effectively emulates the cellular and molecular features of human high-grade squamous intraepithelial lesions.  |  Tuong, ZK., et al. 2018. Papillomavirus Res. 5: 6-20. PMID: 29807614
7. HPV16 E7-Driven Epithelial Hyperplasia Promotes Impaired Antigen Presentation and Regulatory T-Cell Development.  |  Bashaw, AA., et al. 2019. J Invest Dermatol. 139: 2467-2476.e3. PMID: 31207230
8. Evaluation of HPV16 E7 expression in head and neck carcinoma cell lines and clinical specimens.  |  Kitamura, K., et al. 2020. Sci Rep. 10: 22138. PMID: 33335126
9. LncRNA MALAT1 was regulated by HPV16 E7 independently of pRB in cervical cancer cells.  |  Wang, T., et al. 2021. J Cancer. 12: 6344-6355. PMID: 34659524
10. The HPV101 E7 protein shares host cellular targets and biological activities with high-risk HPV16 E7.  |  Gelbard, MK., et al. 2024. Tumour Virus Res. 19: 200300. PMID: 39643241

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HPV16 E7 Antibody (716-325) has 14 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HPV16 E7 Antibody (716-325) Specifications

Alternate Product Name: HPV16 E7

Product Clone Name: 716-325

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: E7

Epitope Species: HPV16

Product Reactivity/Detection: E7

Species Reactivity/Detection: HPV-16

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 14 citations in various scientific publications

Available Conjugates/Forms for HPV16 E7 Antibody (716-325): N/A

The preferred HPV16 E7 antibody is HPV16 E7 Antibody (ED17): sc-6981.