Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
golgin 160 Double Nickase Plasmid (h): sc-403862-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- golgin 160 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- golgin 160 Double Nickase Plasmid (h) and golgin 160 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GOLGA3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: golgin 160 Antibody (C-8): sc-374596
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
golgin 160 Double Nickase Plasmid (h) | sc-403862-NIC | 20 µg | $410.00 | |||
golgin 160 Double Nickase Plasmid (h2) | sc-403862-NIC-2 | 20 µg | $410.00 |
GOLGA3 encodes golgin 160, a coiled-coil golgin localized to the Golgi apparatus that helps organize Golgi architecture and supports vesicle tethering and trafficking between endomembrane compartments. As part of the secretory pathway, golgin 160 contributes to cargo sorting and maintenance of Golgi ribbon integrity, influencing protein processing and delivery to downstream destinations. Disruption of Golgi tethering and trafficking programs is linked to cellular stress responses, altered signaling, and changes in cell polarity and migration, making GOLGA3 a relevant target for studying mechanisms that connect organelle organization with disease-associated phenotypes. In biomedical research, golgin 160 function is often examined in the context of membrane dynamics, secretory pathway regulation, and perturbations of Golgi-dependent proteostasis.
golgin 160 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GOLGA3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GOLGA3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GOLGA3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GOLGA3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.