Ggnbp2 CRISPR/Cas9 KO Plasmid (m): sc-432161
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Ggnbp2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Ggnbp2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ggnbp2 CRISPR/Cas9 KO Plasmid (m) | sc-432161 | 20 µg | $397.00 |
Overview
Ggnbp2 (gametogenetin-binding protein 2) is a germ cell–associated protein implicated in male reproductive biology, with reported enrichment in testicular tissues and links to spermatogenesis-related processes. Although its molecular partners remain incompletely defined, Ggnbp2 has been associated with regulation of transcriptional programs and cellular differentiation states in the germline. Perturbation of genes active in this context can influence chromatin organization, meiotic progression, and sperm development, making Ggnbp2 of interest for studying pathways underlying fertility and germ cell homeostasis. Altered expression patterns of testis-enriched regulators such as Ggnbp2 are also examined in the context of aberrant cell-cycle control and dysregulated differentiation in disease-relevant models.
Ggnbp2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ggnbp2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ggnbp2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ggnbp2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Ggnbp2 protein expression.
This CRISPR knockout system enables efficient generation of Ggnbp2-deficient cell models for investigation of Ggnbp2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ggnbp2 exon(s) critical for Ggnbp2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ggnbp2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Ggnbp2 CRISPR/Cas9 KO Plasmid (m) and Ggnbp2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ggnbp2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Ggnbp2 HDR Plasmid (m) and Ggnbp2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ggnbp2 homology arms to support homology-directed repair at defined Ggnbp2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.