GADD 45γ CRISPR/Cas9 KO Plasmid (h): sc-402483
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GADD 45γ CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GADD 45γ genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GADD 45γ Antibody (B-1): sc-393261
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GADD 45γ CRISPR/Cas9 KO Plasmid (h) | sc-402483 | 20 µg | $397.00 |
Overview
GADD45G (GADD45γ) is a stress-inducible member of the growth arrest and DNA damage–inducible (GADD45) family that links genotoxic and inflammatory cues to cell-cycle control, DNA repair, and apoptosis. It modulates MAPK signaling, including p38 and JNK pathways, and interfaces with checkpoint regulation to promote G2/M arrest and maintenance of genomic stability under cellular stress. In human cells, altered GADD45γ expression has been associated with dysregulated proliferation, impaired stress responses, and aberrant survival signaling in multiple disease contexts, making it a useful node for mechanistic studies of stress adaptation. Its role in integrating environmental stimuli with transcriptional and checkpoint programs supports research into DNA damage responses, senescence, and tumor suppressor–like pathways.
GADD 45γ CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the GADD45G gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the GADD45G together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the GADD45G open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GADD 45γ protein expression.
This CRISPR knockout system enables efficient generation of GADD45G-deficient cell models for investigation of GADD 45γ signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting GADD45G exon(s) critical for GADD 45γ function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple GADD45G genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GADD 45γ CRISPR/Cas9 KO Plasmid (h) and GADD 45γ CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the GADD45G locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GADD 45γ HDR Plasmid (h) and GADD 45γ HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by GADD45G homology arms to support homology-directed repair at defined GADD45G target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.