Date published: 2025-9-9

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Fibronectin Anticorpo (IST-9): sc-59826

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Fichas de dados
  • Fibronectin Anticorpo IST-9é um anticorpo monoclonal produzido em camundongo IgG1 κ, citado em 81 publicações, fornecido em 200 µg/ml
  • uma região no domínio ED-A de cellular Fibronectin de human origem
  • Anti-Fibronectin Anticorpo (IST-9) é recomendado para a detecção de cellular Fibronectin de mouse, rat, human, porcine e canine origem em WB, IP, IF e IHC(P)
  • Anticorpo anti-Fibronectin O anticorpo anti-Fibronectin (IST-9) está disponível conjugado com agarose para IP; HRP para WB, IHC(P) e ELISA; e com fioeritrina ou FITC para IF, IHC(P) e FCM
  • também disponível conjugado com Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 para WB (RGB), IF, IHC(P) e FCM, e para utilização com sistemas de imagiologia fluorescente RGB, tais como iBright™ FL1000, FluorChem™, Typhoon, Azure e outros sistemas comparáveis
  • também disponível conjugado com Alexa Fluor® 680 ou Alexa Fluor® 790 para WB (NIR), IF e FCM; para utilização com sistemas de deteção de infravermelhos próximos (NIR), tais como LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure e outros sistemas comparáveis
  • Entre em contato conosco e receba GRATUITAMENTE um amostra de 10 µg de Fibronectin (IST-9): sc-59826.
  • m-IgG Fc BP-HRP e m-IgG1 BP- HRP são os reagentes de deteção secundários preferidos para Fibronectin Anticorpo (IST-9) para aplicações WB and IHC(P). Estes reagentes são agora oferecidos em conjuntos com Fibronectin Anticorpo (IST-9) (ver informações para encomenda abaixo).

LINKS RÁPIDOS

VEJA TAMBÉM

O anticorpo anti-fibronectina (IST-9) é um anticorpo monoclonal de cadeia leve IgG1 kappa de camundongo que detecta a fibronectina em amostras de camundongo, rato, humano, suíno e canino através de aplicações como western blotting (WB), imunoprecipitação (IP), imunofluorescência (IF) e imunohistoquímica. O anticorpo anti-fibronectina (IST-9) está disponível em formas não conjugadas e em várias formas conjugadas, incluindo agarose, peroxidase de rábano (HRP), ficoeritrina (PE), isotiocianato de fluoresceína (FITC) e vários conjugados Alexa Fluor®. A fibronectina é uma glicoproteína crucial da matriz extracelular que desempenha um papel significativo na adesão celular, motilidade e cicatrização de feridas, tornando a fibronectina vital para a reparação e regeneração dos tecidos. Esta proteína heterodimérica de elevado peso molecular está localizada predominantemente nas superfícies celulares, nos fluidos extracelulares e no plasma, onde a fibronectina facilita as interações entre as células e a matriz extracelular. A emenda alternativa e a glicosilação da fibronectina levam à geração de várias isoformas, algumas das quais estão associadas a tecidos específicos ou a condições patológicas, como as malignidades. A presença de Fibronectina glicosilada e de variantes de emenda da Fibronectina tem sido associada à formação do fenótipo de miofibroblasto na doença de Dupuytren, realçando a importância de compreender as modificações pós-traducionais em contextos de doença.

Para uso em exclusivo em pesquisa. Não se destina a uso em diagnostico e tratamento.

Alexa Fluor® é uma marca comercial da Molecular Probes Inc., OR., EUA

LI-COR® e Odyssey® são marcas registadas da LI-COR Biosciences

Informacoes sobre ordens

Nome do ProdutoNumero de CatalogoUNIDPrecoQdeFAVORITOS

Fibronectin Anticorpo (IST-9)

sc-59826
200 µg/ml
$316.00

Pacote do Fibronectin (IST-9): m-IgG Fc BP-HRP

sc-526943
200 µg Ab; 10 µg BP
$354.00

Pacote do Fibronectin (IST-9): m-IgG1 BP-HRP

sc-532316
200 µg Ab; 20 µg BP
$354.00

Fibronectin Anticorpo (IST-9) AC

sc-59826 AC
500 µg/ml, 25% agarose
$416.00

Fibronectin Anticorpo (IST-9) HRP

sc-59826 HRP
200 µg/ml
$316.00

Fibronectin Anticorpo (IST-9) FITC

sc-59826 FITC
200 µg/ml
$330.00

Fibronectin Anticorpo (IST-9) PE

sc-59826 PE
200 µg/ml
$343.00

Fibronectin Anticorpo (IST-9) Alexa Fluor® 488

sc-59826 AF488
200 µg/ml
$357.00

Fibronectin Anticorpo (IST-9) Alexa Fluor® 546

sc-59826 AF546
200 µg/ml
$357.00

Fibronectin Anticorpo (IST-9) Alexa Fluor® 594

sc-59826 AF594
200 µg/ml
$357.00

Fibronectin Anticorpo (IST-9) Alexa Fluor® 647

sc-59826 AF647
200 µg/ml
$357.00

Fibronectin Anticorpo (IST-9) Alexa Fluor® 680

sc-59826 AF680
200 µg/ml
$357.00

Fibronectin Anticorpo (IST-9) Alexa Fluor® 790

sc-59826 AF790
200 µg/ml
$357.00

For IST-9 there is a picture of kidney tissue stained for IHC using IST-9 antibody. Can you please give me the reference for the image as i am looking for the IHC protocol used to produce this image i.e. processing and antigen optimisation process used.

Asked by: Pvirdee
Thanks for your question. The image is the results of in-house validation by Immunoperoxidase staining of formalin fixed, paraffin-embedded. You can find our protocol here https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining . Please contact technical service for further support.
Answered by: SCBT Heidelberg Support
Date published: 2022-11-09

Hi Can I get a free sample of this antibody. (Fibronectin Antibody (IST-9): sc-59826) I wanted to see if it works with human sample for western blot. thank you Sindhu Saraswathy PhD Scientist Doheny Eye Institute

Asked by: Sindhu Saraswathy
Thank you for your question. Please contact Technical Service for more information on how to receive a 10 μg sample of our well characterized monoclonal antibody.
Answered by: Technical Support
Date published: 2018-12-05

Does this antibody work for IF of formalin-fixed paraffin-embedded tissue?

Asked by: PhD Student
Thank you for your question. Yes, this antibody is recommended for IF and IHC(P) and will work perfectly fine for IF on formalin fixed tissues. Please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Tech Service
Date published: 2017-01-10

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: DefinitelyNotMatt
Thank you for your question. For IHC, we recommend using a directly conjugated mouse monoclonal primary antibody. We recommend using SC-8422 for Fibronectin antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. Please contact Technical Service if you have further questions concerning this product.
Answered by: Tech Service 9
Date published: 2016-12-30
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Rated 2 out of 5 by from no bandI used this antibody at 1:200 - 1:1000 as 1st antibody with 2nd mouse antibody at 1:1000 - 1:5000. Both 1st and 2nd antibody I dilute with 5% skim milk but still doesn't work. even on semi-dry or wet transfer also showed no band. Any kind suggestion please?
Date published: 2024-03-25
Rated 4 out of 5 by from Great productIt is good antibodies in WB. It works in dilution 1:1000
Date published: 2018-07-14
Rated 2 out of 5 by from Strong background in IF on rat frozen liverRat liver frozen sections were subjected to immunofluorescence using Fibronectin (IST-9) antibody. Antigen retrieval methods were optimized between without antigen retrieval, heat-induced citrate buffer, and trypsin-induced antigen retrieval. Some signals could be observed in portal tracts, but background was strong.
Date published: 2018-03-14
Rated 4 out of 5 by from Works wellI received free samples and tried on western blotting. Tha bands are thick with no secondary bands. Need to dilute antibody concentrations to get good data.
Date published: 2018-02-12
Rated 5 out of 5 by from So nice!I received this Ab in mid-November 2017 and was very pleased to see it worked nicely! Will definitely purchase it...
Date published: 2018-01-13
Rated 3 out of 5 by from Staining of distal colon for fibronectin EDAI've had the chance to test the antibody for the staining of fibronectin EDA in the distal colon of mice that were developing fibrosis in the colon. Unfortunately, we did not see staining of the paraffin embedded slides, which also means that there is no background staining and the antibody is very specific. We are currently still trying to change some aspects of the staining protocol to check if we can get better staining.
Date published: 2017-12-04
Rated 5 out of 5 by from clear bandThis is a great antibody, the band was clear and significant.
Date published: 2017-05-13
Rated 5 out of 5 by from The band is clearlyWe detected positive signal in WB using mouse lung, strong signal;
Date published: 2017-04-22
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Fibronectin Anticorpo (IST-9) is rated 4.2 out of 5 by 13.
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