Fibrillarin Antibody (H-9): sc-514239

Fibrillarin Antibody (H-9) is a mouse monoclonal IgM antibody that detects fibrillarin in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Fibrillarin is a crucial protein predominantly localized in the fibrillar region of the nucleolus, specifically within the dense fibrillar and fibrillar center regions, where fibrillarin plays a vital role in processing ribosomal RNA (rRNA) transcripts. This localization underscores fibrillarin′s involvement in ribosome biogenesis, a fundamental process for cellular function and growth. Fibrillarin is expressed in various cell types, including HeLa cells, 3T3 cells, and human peripheral blood lymphocytes, and appears at chromosomal nucleolar regions during metaphase and anaphase, marking fibrillarin′s importance in cell division. During telophase, fibrillarin serves as an early marker for new nucleolus formation, highlighting fibrillarin′s dynamic role in cellular organization. Fibrillarin′s structure features an RNA-binding domain and an RNP consensus sequence, which facilitates interaction with U3 small nucleolar RNA, further emphasizing fibrillarin′s essential function in rRNA processing and ribosome assembly. Anti-fibrillarin antibody (H-9) is an invaluable tool for researchers studying nucleolar dynamics and ribosome biogenesis in various biological contexts.

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Fibrillarin Antibody (H-9) References:

1. Double immunolocalization of major nucleolar proteins, fibrillarin and B23, in dividing mammalian cultured cells.  |  Mukharyamova, KS., et al. 1999. Membr Cell Biol. 12: 829-43. PMID: 10512052
2. Arginine methylation of recombinant murine fibrillarin by protein arginine methyltransferase.  |  Lin, CH., et al. 2002. J Protein Chem. 21: 447-53. PMID: 12523648
3. Structural and thermodynamic evidence for a stabilizing role of Nop5p in S-adenosyl-L-methionine binding to fibrillarin.  |  Aittaleb, M., et al. 2004. J Biol Chem. 279: 41822-9. PMID: 15286083
4. Fibrillarin and Nop56 interact before being co-assembled in box C/D snoRNPs.  |  Lechertier, T., et al. 2009. Exp Cell Res. 315: 928-42. PMID: 19331828
5. Analysis of nucleolar protein fibrillarin mobility and functional state in living HeLa cells.  |  Barygina, VV., et al. 2010. Biochemistry (Mosc). 75: 979-88. PMID: 21073418
6. The Nucleolar Fibrillarin Protein Is Required for Helper Virus-Independent Long-Distance Trafficking of a Subviral Satellite RNA in Plants.  |  Chang, CH., et al. 2016. Plant Cell. 28: 2586-2602. PMID: 27702772
7. Differential expression of the nucleolar protein fibrillarin during mammalian spermatogenesis and its probable association with chromatoid body components.  |  de Pauli, LF., et al. 2017. Micron. 94: 37-45. PMID: 28027486
8. Identification of Functional Domain(s) of Fibrillarin Interacted with p2 of Rice stripe virus.  |  Zheng, L., et al. 2018. Can J Infect Dis Med Microbiol. 2018: 8402839. PMID: 29736196
9. Atypical chemokine receptor 3 induces colorectal tumorigenesis in mice by promoting β-arrestin-NOLC1-fibrillarin-dependent rRNA biogenesis.  |  Yang, J., et al. 2022. Acta Pharmacol Sin. 43: 2967-2976. PMID: 35365782
10. Nucleolin and fibrillarin expression in stimulated lymphocytes and differentiating HL-60 cells. A flow cytometric assay.  |  Méhes, G. and Pajor, L. 1995. Cell Prolif. 28: 329-36. PMID: 7626687

The preferred Fibrillarin antibody is Fibrillarin Antibody (B-1): sc-166001.