Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
eIF4AI CRISPR/Cas9 KO Plasmid (m): sc-420146
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- eIF4AI CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the eIF4AI genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
eIF4AI CRISPR/Cas9 KO Plasmid (m) | sc-420146 | 20 µg | $397.00 |
Overview
Eif4a1 encodes the mouse eIF4AI DEAD-box RNA helicase, a core component of the eIF4F translation initiation machinery that unwinds structured 5′ UTRs to enable ribosome scanning and start-codon recognition. Through ATP-dependent remodeling of mRNA secondary structure, eIF4AI supports cap-dependent translation and influences proteostasis, stress adaptation, and cell-cycle progression. Its activity integrates with mTOR-regulated control of protein synthesis and can shape selective translation programs affecting growth, metabolism, and differentiation. Altered translation initiation dynamics involving eIF4A-family helicases have been linked to oncogenic signaling, neurodevelopmental phenotypes, and stress-response dysregulation, making Eif4a1 a relevant node for mechanistic studies of translational control.
eIF4AI CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Eif4a1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Eif4a1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Eif4a1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish eIF4AI protein expression.
This CRISPR knockout system enables efficient generation of Eif4a1-deficient cell models for investigation of eIF4AI signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Eif4a1 exon(s) critical for eIF4AI function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Eif4a1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by eIF4AI CRISPR/Cas9 KO Plasmid (m) and eIF4AI CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Eif4a1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by eIF4AI HDR Plasmid (m) and eIF4AI HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Eif4a1 homology arms to support homology-directed repair at defined Eif4a1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.