eIF1 CRISPR/Cas9 KO Plasmid (m): sc-423216
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- eIF1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the eIF1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
eIF1 CRISPR/Cas9 KO Plasmid (m) | sc-423216 | 20 µg | $397.00 |
Overview
Mouse Eif1 encodes eukaryotic translation initiation factor 1 (eIF1), a conserved component of the 43S preinitiation complex that promotes accurate start-codon selection during cap-dependent translation initiation. By enforcing scanning stringency and stabilizing an open conformation of the 40S subunit, eIF1 helps control global protein synthesis and proteome fidelity. This function links Eif1 to translational control programs that shape cell growth, stress adaptation, and differentiation through mRNA-selective initiation. Disruption of translation initiation fidelity is broadly relevant to disease-associated states characterized by altered proteostasis and aberrant growth signaling, making Eif1 a useful node for mechanistic studies of translational dysregulation.
eIF1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Eif1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Eif1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Eif1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish eIF1 protein expression.
This CRISPR knockout system enables efficient generation of Eif1-deficient cell models for investigation of eIF1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Eif1 exon(s) critical for eIF1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Eif1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by eIF1 CRISPR/Cas9 KO Plasmid (m) and eIF1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Eif1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by eIF1 HDR Plasmid (m) and eIF1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Eif1 homology arms to support homology-directed repair at defined Eif1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.