CYPOR Antibody (G-5): sc-25263

CYPOR Antibody (G-5) is a mouse monoclonal IgG2a light chain antibody that detects CYPOR in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CYPOR, also known as cytochrome P450 reductase, plays a crucial role in the metabolism of various endogenous and xenobiotic compounds by facilitating the transfer of electrons from NADPH to cytochrome P450 enzymes during their catalytic cycle. This electron transfer is essential for the proper functioning of the P450 enzymes, which are involved in the biotransformation of drugs and the synthesis of steroid hormones, making CYPOR vital for drug metabolism and detoxification processes. Located in the endoplasmic reticulum, CYPOR is not only responsible for supporting cytochrome P450 enzymes but also transfers electrons to heme oxygenase and cytochrome b5, highlighting CYPOR′s importance in multiple metabolic pathways. Structurally, CYPOR is related to two flavoprotein families, ferredoxin nucleotide reductase and flavodoxin, and CYPOR′s electron transfer activity relies on the binding of two flavin cofactors, FAD and FMN, to respective domains. CYPOR Antibody (G-5) is an invaluable tool for researchers studying metabolic processes and the interactions of cytochrome P450 enzymes in human biology.

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CYPOR Antibody (G-5) References:

1. Cytochrome P450 reductase-mediated anaerobic biotransformation of ethanol to 1-hydroxyethyl-free radicals and acetaldehyde.  |  Díaz Gómez, MI., et al. 2000. Toxicology. 154: 113-22. PMID: 11118675
2. Electron transfer in human cytochrome P450 reductase.  |  Gutierrez, A., et al. 2003. Biochem Soc Trans. 31: 497-501. PMID: 12773143
3. The interaction of microsomal cytochrome P450 2B4 with its redox partners, cytochrome P450 reductase and cytochrome b(5).  |  Im, SC. and Waskell, L. 2011. Arch Biochem Biophys. 507: 144-53. PMID: 21055385
4. Regulation of gap junction function and Connexin 43 expression by cytochrome P450 oxidoreductase (CYPOR).  |  Polusani, SR., et al. 2011. Biochem Biophys Res Commun. 411: 490-5. PMID: 21726529
5. 7-Dehydrocholesterol reductase activity is independent of cytochrome P450 reductase.  |  Zou, L., et al. 2011. J Steroid Biochem Mol Biol. 127: 435-8. PMID: 21762780
6. Suppression of cytochrome P450 reductase expression promotes astrocytosis in subventricular zone of adult mice.  |  Yao, Y., et al. 2013. Neurosci Lett. 548: 84-9. PMID: 23727388
7. Solution structure of the cytochrome P450 reductase-cytochrome c complex determined by neutron scattering.  |  Freeman, SL., et al. 2018. J Biol Chem. 293: 5210-5219. PMID: 29475945
8. NADPH-cytochrome P450 reductase expression and enzymatic activity in primary-like human hepatocytes and HepG2 cells for in vitro biotransformation studies.  |  Schulz, C., et al. 2019. Clin Hemorheol Microcirc. 73: 249-260. PMID: 31561354
9. Exploring Novel Variants of the Cytochrome P450 Reductase Gene (POR) from the Genome Aggregation Database by Integrating Bioinformatic Tools and Functional Assays.  |  Rojas Velazquez, MN., et al. 2023. Biomolecules. 13: PMID: 38136599
10. A flexible linker of 8-amino acids between the membrane binding segment and the FMN domain of cytochrome P450 reductase is necessary for optimal activity.  |  Rwere, F., et al. 2024. J Inorg Biochem. 259: 112667. PMID: 39032346

The preferred CYPOR antibody is CYPOR Antibody (F-10): sc-25270.