Date published: 2025-9-7

00800 4573 8000

SCBT Portrait Logo
Seach Input

cyclin A Anticorpo (B-8): sc-271682

4.5(35)
Escrever uma avaliaçãoFazer uma pergunta

Fichas de dados
  • cyclin A Anticorpo B-8é um anticorpo monoclonal produzido em camundongo IgG2a κ cyclin A anticorpo, citado em 132 publicações, fornecido em 200 µg/ml
  • Produzido contra aminoácidos 1-432 que representam a completa extensão cyclin A de human origem
  • cyclin A Anticorpo (B-8) é recomendado para a detecção de cyclin A and cyclin A1 of human origin by WB, IP, IF, IHC(P) and ELISA
  • Anticorpo anti-cyclin A O anticorpo anti-cyclin A (B-8) está disponível conjugado com agarose para IP; HRP para WB, IHC(P) e ELISA; e com fioeritrina ou FITC para IF, IHC(P) e FCM
  • também disponível conjugado com Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 para WB (RGB), IF, IHC(P) e FCM, e para utilização com sistemas de imagiologia fluorescente RGB, tais como iBright™ FL1000, FluorChem™, Typhoon, Azure e outros sistemas comparáveis
  • também disponível conjugado com Alexa Fluor® 680 ou Alexa Fluor® 790 para WB (NIR), IF e FCM; para utilização com sistemas de deteção de infravermelhos próximos (NIR), tais como LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure e outros sistemas comparáveis
  • dispnonível como reagente TransCruz para o uso em ChIP (sc-271682 X, 200 µg/0.1 ml)
  • Entre em contato conosco e receba GRATUITAMENTE um amostra de 10 µg de cyclin A (B-8): sc-271682.
  • m-IgG Fc BP-HRP e 2a BP-HRP">m-IgG2a BP-HRP são os reagentes de deteção secundários preferidos para cyclin A Antibody (B-8) para aplicações WB e IHC(P). Estes reagentes são agora oferecidos em conjuntos com cyclin A Antibody (B-8)(ver informações de encomenda abaixo).

LINKS RÁPIDOS

VEJA TAMBÉM

O Anticorpo ciclina A (B-8) é um anticorpo monoclonal de camundongo IgG2a κ para ciclina A (também designado anticorpo CCNA2) que detecta a proteína ciclina A de origem humana por WB, IP, IF, IHC(P) e ELISA. O anticorpo ciclina A (B-8) está disponível na forma de anticorpo anticiclina A não conjugado, bem como em várias formas conjugadas de anticorpo anticiclina A, incluindo agarose, HRP, PE, FITC e vários conjugados Alexa Fluor®. A função crítica que a família de proteínas reguladoras conhecidas como ciclinas desempenha na regulação do ciclo celular eucariótico está bem estabelecida. O complexo de ciclina mais bem caracterizado é a ciclina mitótica B/Cdc2 p34 quinase, o componente ativado do fator promotor de maturação. A ciclina A se acumula antes da ciclina B no ciclo celular, parece estar envolvida no controle da fase S e mostrou que podem ser associadas à quinase-2 dependente de ciclina (Cdk2). Além disso, a ciclina A tem sido implicada na transformação celular e é encontrada em complexos com E1A, fatores de transcrição DRTF1 e E2F e proteína de retinoblastoma, p110. Uma segunda forma de ciclina A, denominada ciclina A1 devido à sua alta homologia de sequência com a ciclina A1 de Xenopus, é mais altamente expressa em células germinativas. Foi proposto que a ciclina A1 pode se associar a Cdk2, p39 e Cdc2 p34.

Para uso em exclusivo em pesquisa. Não se destina a uso em diagnostico e tratamento.

Alexa Fluor® é uma marca comercial da Molecular Probes Inc., OR., EUA

LI-COR® e Odyssey® são marcas registadas da LI-COR Biosciences

Informacoes sobre ordens

Nome do ProdutoNumero de CatalogoUNIDPrecoQdeFAVORITOS

cyclin A Anticorpo (B-8)

sc-271682
200 µg/ml
$316.00

Pacote do cyclin A (B-8): m-IgG Fc BP-HRP

sc-525820
200 µg Ab; 10 µg BP
$354.00

Pacote do cyclin A (B-8): m-IgG2a BP-HRP

sc-546429
200 µg Ab; 10 µg BP
$354.00

cyclin A Anticorpo (B-8) X

sc-271682 X
200 µg/0.1 ml
$316.00

cyclin A Anticorpo (B-8) AC

sc-271682 AC
500 µg/ml, 25% agarose
$416.00

cyclin A Anticorpo (B-8) HRP

sc-271682 HRP
200 µg/ml
$316.00

cyclin A Anticorpo (B-8) FITC

sc-271682 FITC
200 µg/ml
$330.00

cyclin A Anticorpo (B-8) PE

sc-271682 PE
200 µg/ml
$343.00

cyclin A Anticorpo (B-8) Alexa Fluor® 488

sc-271682 AF488
200 µg/ml
$357.00

cyclin A Anticorpo (B-8) Alexa Fluor® 546

sc-271682 AF546
200 µg/ml
$357.00

cyclin A Anticorpo (B-8) Alexa Fluor® 594

sc-271682 AF594
200 µg/ml
$357.00

cyclin A Anticorpo (B-8) Alexa Fluor® 647

sc-271682 AF647
200 µg/ml
$357.00

cyclin A Anticorpo (B-8) Alexa Fluor® 680

sc-271682 AF680
200 µg/ml
$357.00

cyclin A Anticorpo (B-8) Alexa Fluor® 790

sc-271682 AF790
200 µg/ml
$357.00

Hey there! I'm interested in purchasing one of your Cyclin A-Alexa Fluor (Anti-cyclin A Antibody (B-8): sc-271682) conjugates and have a question. Are these antibody fusions recognized by other anti-mouse alexa fluor secondary antibodies? Thank you!

Asked by: Mike F
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-04-29

Does it work for IF using Rat or Mouse tissue?

Asked by: Tiago D
Thank you for your question. This antibody has not been tested for use on any other species than human samples, so we only recommend and warranty it for use on human samples. It may work in other species, but we can not warranty it for that use. Please contact our Technical Service Department by phone, e-mail or live chat if you have any further questions.
Answered by: Tech Service
Date published: 2017-04-26

I need to use an antibody to detect the protein in samples of canine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 3
Date published: 2017-01-12

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with cyclin A (B-8): sc-271682?

Asked by: jerojero
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 3
Date published: 2017-01-12
  • y_2025, m_8, d_27, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_4
  • loc_pt_PT, sid_271682, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 98ms
  • QUESTIONS, PRODUCT
Rated 1 out of 5 by from New antibody lot doesn't perform well anymoreUnfortunately, after repurchasing the antibody it did not perform as previous lot. I have used old lot for IF at 1:500 dilution in human RPE1 cells successfully and got a specific nuclear staining. However, when I purchased the antibody again it was totally non-specific and resulted mainly in cytoplasmic localisation. I have contacted Santa Cruz support and they sent me a replacement, but even the replacement was not performing as the old antibody lot. It is very disappointing that different antibody lots are not reproducible.
Date published: 2022-09-15
Rated 5 out of 5 by from WB results刚才没看货号就评价了,尴尬!我们用的是这个货号,免疫印迹实验结果非常好,1:1000就可以做出来,或许与样品来源相关,强烈推荐啊。
Date published: 2020-11-15
Rated 4 out of 5 by from Works well for western blot and IPI have used this antibody for WB 1:500. It recognises a band at 54 kDa. However it also detects a band around 102 kDa. It also perform well for IP, 2 ug with 500ug lysate for RPE1 cells 2h RT. Looking forward to test if for IF.
Date published: 2020-09-30
Rated 5 out of 5 by from 免疫沈降で使用Immunoprecipitationで使用。5ul/sampleで用いた。非常に良好に免疫沈降することができた。
Date published: 2020-06-19
Rated 4 out of 5 by from Good for immunofluorescent stainingThe antibody can give a specific staining profile matches with Cyclin A with 1:500 dilution. Samples were HeLa cells fixed with cold methanol.
Date published: 2019-10-08
Rated 4 out of 5 by from ok on WBAntibody detect a specific band at 50Kda on human cheratinocytes extracts
Date published: 2018-12-12
Rated 5 out of 5 by from good productIt is a good antibody with clean background for WB.
Date published: 2018-09-11
Rated 5 out of 5 by from Works well in IFWorks well in IF in blocking buffer (BSA 3%, Triton 0.1%). I used it at 1/200
Date published: 2018-07-16
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_35
  • loc_pt_PT, sid_271682, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 10ms
  • REVIEWS, PRODUCT
cyclin A Anticorpo (B-8) is rated 4.5 out of 5 by 35.
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_35
  • loc_pt_PT, sid_271682, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 99ms
  • REVIEWS, PRODUCT