CIITA (m2): 293T Lysate: sc-178403

CIITA (m2): 293T Lysate is derived from the 293T cell line, a human embryonic kidney cell line engineered to express the SV40 large T antigen, which enhances its transfection efficiency and protein production capabilities. This lysate is specifically enriched with mouse Class II Transactivator (CIITA), a master regulator of Major Histocompatibility Complex (MHC) class II gene expression. CIITA acts as a transcriptional coactivator that does not bind DNA directly but is recruited to MHC class II promoters through its interaction with DNA-binding proteins and other transcription factors. In research, CIITA (m2): 293T Lysate is utilized to study the regulatory mechanisms of MHC class II gene expression and the role of CIITA in immune response modulation. Researchers employ this lysate in Western blotting to detect and quantify CIITA expression under various experimental conditions, providing insights into its regulation and activity. The lysate is also used in chromatin immunoprecipitation (ChIP) assays to investigate the recruitment of CIITA to MHC class II promoters and to understand its role in chromatin remodeling and transcriptional activation. Additionally, co-immunoprecipitation assays using CIITA (m2): 293T Lysate help identify protein-protein interactions involving CIITA, elucidating its role in the assembly of transcriptional complexes. Functional assays can assess the impact of CIITA on the transcriptional activity of MHC class II genes, contributing to a deeper understanding of its role in antigen presentation and immune regulation.