Cdk4 Antibody (DCS-31): sc-56277

Cdk4 Antibody (DCS-31) is a mouse monoclonal IgG2a kappa light chain antibody specifically engineered to detect Cyclin-dependent kinase 4 (Cdk4), also known as CDC2-like kinase 4, in mouse, rat, and human samples. anti-Cdk4 antibody (DCS-31) recognizes the full-length human Cdk4 protein, making it suitable for applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Cdk4 is a pivotal kinase located primarily in the nucleus and cytoplasm, where it forms complexes with D-type cyclins to regulate the progression of the cell cycle from the G1 to the S phase. By phosphorylating key substrates like the retinoblastoma protein (Rb), Cdk4 controls essential transitions that drive cell division, underscoring its importance in cellular proliferation and cancer research. The activity of Cdk4 is finely tuned through interactions with cyclins, which enhance its kinase activity, and inhibitors such as p16, which suppress its function. Additionally, the activation of Cdk4-cyclin complexes involves phosphorylation by Cdk-activating proteins (CAK), highlighting the sophisticated regulatory mechanisms that ensure proper cell cycle progression. Cdk4 monoclonal antibody (DCS-31) is available in both non-conjugated form and a variety of conjugated formats, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, to accommodate diverse experimental needs. Cdk4 (DCS-31) antibody is an invaluable tool for researchers investigating cell cycle dynamics and related signaling pathways.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Cdk4 Antibody (DCS-31) has 52 citations in a variety of scientific publications.

Primary antibodies like Anti-Cdk4 Antibody (DCS-31) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Cdk4 Antibody (DCS-31) Specifications

Alternate Product Name: Cdk4

Product Clone Name: DCS-31

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-303

Epitope Target: Cdk4

Epitope Species: human

Product Reactivity/Detection: Cdk4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 52 citations in various scientific publications

Available Conjugates/Forms for Cdk4 Antibody (DCS-31): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Cdk4 antibody is Cdk4 Antibody (DCS-35): sc-23896.