Cdc34B Antibody (E-6): sc-376097

Cdc34B Antibody (E-6) is a mouse monoclonal IgG2a kappa light chain antibody that detects Cdc34B in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Cdc34B antibody (E-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Cdc34B, also known as UBE2R2 or UBC3B, is a 238 amino acid protein that belongs to the E2 ubiquitin-conjugating enzyme family, playing a crucial role in the ubiquitin-proteasome pathway. Cdc34B is primarily located in the cytoplasm and is essential for the regulation of protein degradation, which is vital for maintaining cellular homeostasis and facilitating cell cycle progression. Cdc34B catalyzes the attachment of ubiquitin to target proteins, such as β-TrCP, an F-box protein that mediates the degradation of β-catenin, a key regulator in the Wnt signaling pathway. The phosphorylation of Cdc34B by casein kinase II enhances recognition of β-TrCP substrates, thereby promoting β-catenin degradation and influencing cell cycle dynamics. Given Cdc34B′s significant role in regulating protein turnover and cell cycle progression, Cdc34B monoclonal antibody (E-6) serves as an invaluable tool for researchers studying these processes.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Cdc34B Antibody (E-6) References:

1. Control of methionine biosynthesis genes by protein kinase CK2-mediated phosphorylation of Cdc34.  |  Barz, T., et al. 2006. Cell Mol Life Sci. 63: 2183-90. PMID: 16952051
2. Ubiquitin-proteasome system as a factor that determine the sensitivity to methylmercury.  |  Hwang, GW. 2007. Yakugaku Zasshi. 127: 463-8. PMID: 17329932
3. cdc25 is a specific tyrosine phosphatase that directly activates p34cdc2.  |  Gautier, J., et al. 1991. Cell. 67: 197-211. PMID: 1913817
4. Mitotic transmission of artificial chromosomes in cdc mutants of the yeast, Saccharomyces cerevisiae.  |  Palmer, RE., et al. 1990. Genetics. 125: 763-74. PMID: 2204582
5. A new twist to the cell cycle.  |  Barinaga, M. 1995. Science. 269: 631-2. PMID: 7624789
6. A 20S complex containing CDC27 and CDC16 catalyzes the mitosis-specific conjugation of ubiquitin to cyclin B.  |  King, RW., et al. 1995. Cell. 81: 279-88. PMID: 7736580
7. Cloning of the human homolog of the CDC34 cell cycle gene by complementation in yeast.  |  Plon, SE., et al. 1993. Proc Natl Acad Sci U S A. 90: 10484-8. PMID: 8248134
8. Mammalian p50Cdc37 is a protein kinase-targeting subunit of Hsp90 that binds and stabilizes Cdk4.  |  Stepanova, L., et al. 1996. Genes Dev. 10: 1491-502. PMID: 8666233
9. A human protein related to yeast Cdc6p.  |  Williams, RS., et al. 1997. Proc Natl Acad Sci U S A. 94: 142-7. PMID: 8990175

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Cdc34B Antibody (E-6) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-Cdc34B Antibody (E-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Cdc34B Antibody (E-6) Specifications

Alternate Product Name: Cdc34B

Product Clone Name: E-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 188-238

Epitope Target: Cdc34B

Epitope Species: human

Product Reactivity/Detection: Cdc34B

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Cdc34B Antibody (E-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent