Date published: 2025-9-7

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Aquaporin 4/AQP4 Anticorpo (4/18): sc-32739

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Fichas de dados
  • Aquaporin 4/AQP4 Anticorpo 4/18é um anticorpo monoclonal produzido em camundongo IgG3 κ Aquaporin 4/AQP4 anticorpo, citado em 43 publicações, fornecido em 200 µg/ml
  • Produzido contra aminoácidos 301-318 de intracellular AQP4 de human origem
  • recomendado para a detecção de AQP4 de mouse, rat e human origem em
  • disponível na forma conjugada à agarose para IP; ficoeritrina, FITC, Alexa Fluor® 488 ou Alexa Fluor® 647 para IF, IHC(P) e FCM
  • Entre em contato conosco e receba GRATUITAMENTE um amostra de 10 µg de Aquaporin 4/AQP4 (4/18): sc-32739.
  • Atualmente, ainda não concluímos a identificação do(s) reagente(s) de deteção secundário(s) preferido(s) para Aquaporin 4/AQP4 Anticorpo (4/18). Este trabalho está em curso.

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VEJA TAMBÉM

O anticorpo anti-AQP4 (4/18) é um anticorpo monoclonal IgG3 de cadeia leve kappa de ratinho que detecta a proteína AQP4 de origem de camundongo, rato e humana por western blotting (WB), imunoprecipitação (IP), imunofluorescência (IF), imunohistoquímica e citometria de fluxo (FCM). O anticorpo anti-AQP4 (4/18) está disponível em formas não conjugadas e em várias formas conjugadas, incluindo agarose, ficoeritrina (PE), isotiocianato de fluoresceína (FITC) e vários conjugados Alexa Fluor®. A AQP4, também conhecida como aquaporina 4, desempenha um papel crucial no transporte de água através das membranas celulares, particularmente no sistema nervoso central e no músculo esquelético. A AQP4 está predominantemente localizada no sarcolema das fibras musculares de contração rápida, onde a AQP4 facilita o movimento rápido da água, essencial para a função muscular e a hidratação. Esta localização ajuda a manter o equilíbrio osmótico e apoia a contração muscular durante a atividade física intensa. As aquaporinas, incluindo a AQP4, são proteínas integrais da membrana que formam canais que permitem a permeabilidade selectiva à água, e a disfunção das aquaporinas pode levar a várias condições fisiopatológicas, incluindo edema e distúrbios neurológicos. A presença de múltiplas isoformas de aquaporina nos tecidos sublinha a sua importância em diversos processos fisiológicos, como a absorção renal de água e a regulação da dinâmica do fluido cerebrospinal.

Para uso em exclusivo em pesquisa. Não se destina a uso em diagnostico e tratamento.

Alexa Fluor® é uma marca comercial da Molecular Probes Inc., OR., EUA

LI-COR® e Odyssey® são marcas registadas da LI-COR Biosciences

Referencias do Aquaporin 4/AQP4 Anticorpo (4/18):

  1. A família de canais de água aquaporina de mamíferos: Um novo e promissor alvo terapêutico.  |  Beitz, E. and Schultz, JE. 1999. Curr Med Chem. 6: 457-67. PMID: 10213793
  2. Isolamento do cDNA para a proteína de membrana integral de eritrócitos de 28 kilodaltons: membro de uma antiga família de canais.  |  Preston, GM. and Agre, P. 1991. Proc Natl Acad Sci U S A. 88: 11110-4. PMID: 1722319
  3. Estrutura e localização cromossómica do gene de um canal de água humano (AQP3).  |  Ishibashi, K., et al. 1995. Genomics. 27: 352-4. PMID: 7558005
  4. Clonagem do cDNA, organização do gene e localização cromossómica de um canal de água humano insensível ao mercúrio. Evidência de unidades transcricionais distintas.  |  Yang, B., et al. 1995. J Biol Chem. 270: 22907-13. PMID: 7559426
  5. Necessidade do canal de água renal humano aquaporina-2 para a concentração de urina dependente da vasopressina.  |  Deen, PM., et al. 1994. Science. 264: 92-5. PMID: 8140421
  6. O gene da aquaporina-5 humana. Caracterização molecular e localização cromossómica.  |  Lee, MD., et al. 1996. J Biol Chem. 271: 8599-604. PMID: 8621489
  7. Clonagem e localização cromossómica da aquaporina 4 do rato: exclusão de um fenótipo mutante candidato, a ataxia.  |  Turtzo, LC., et al. 1997. Genomics. 41: 267-70. PMID: 9143504
  8. Expressão da aquaporina-4 em fibras de contração rápida do músculo esquelético de mamíferos.  |  Frigeri, A., et al. 1998. J Clin Invest. 102: 695-703. PMID: 9710437
  9. Aquaporinas.  |  Echevarría, M. and Ilundáin, AA. 1998. J Physiol Biochem. 54: 107-18. PMID: 9858131

Informacoes sobre ordens

Nome do ProdutoNumero de CatalogoUNIDPrecoQdeFAVORITOS

Aquaporin 4/AQP4 Anticorpo (4/18)

sc-32739
200 µg/ml
$316.00

Aquaporin 4/AQP4 Anticorpo (4/18) AC

sc-32739 AC
500 µg/ml, 25% agarose
$416.00

Aquaporin 4/AQP4 Anticorpo (4/18) Alexa Fluor® 488

sc-32739 AF488
200 µg/ml
$357.00

Aquaporin 4/AQP4 Anticorpo (4/18) Alexa Fluor® 647

sc-32739 AF647
200 µg/ml
$357.00

Aquaporin 4/AQP4 Anticorpo (4/18) FITC

sc-32739 FITC
200 µg/ml
$330.00

Aquaporin 4/AQP4 Anticorpo (4/18) PE

sc-32739 PE
200 µg/ml
$343.00

Hi, does this antibody require permeabilization for staining astrocytes by flow?

Asked by: JJY2
Thank you for your question. Please see permeabilzation steps in our protocol on section C : https://www.scbt.com/resources/protocols/flow-cytometry
Answered by: BlakeJ
Date published: 2024-11-05

I am currently using your AQP4 antibody (sc-32739) in western blots and found that ECL results showed bands at ~70kDa and fluorescence showed bands at ~35 kDa. Do you know why this might be the case?

Asked by: greeny5
Thank you for your question. For assistance please contact technical service by email scbt@scbt.com or phone (800)-457-3801.
Answered by: BlakeJ
Date published: 2023-06-07

I am trying IHC on mouse tissue sections. Which secondary antibody would you recommend? H+L or IgG3 specific? This antibody also gives a lot of bands in western blot around 40 kDa, not exactly around the expected size of the AQP4 isoforms.

Asked by: Tejal A
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-04-07

I am a PhD student at the university of Washington. We are looking for a good antibody for mouse kidney and brain sections and wondering if you could offer a free aliquot of AQP4 Antibody (sc-32739) for us to test first.

Asked by: Chenyi
Thank you for your question. Please contact your local distributor or technical service for further assistance.
Answered by: Dallas Tech Service
Date published: 2021-04-20

This is from Johns hopkins University. We plan to order AQP4 Antibody (4/18): sc-32739. First we want to make sure if the antibody can arrived tomorrow if we ordered today? Thanks!

Asked by: Anonymous
Thank you for your question. Yes, we ship all our antibody reagents overnight, so if you order a vial of sc-32739 today, you will receive it tomorrow. It is currently in stock and ready to ship to you!
Answered by: Tech Service
Date published: 2019-11-11

We used it to test for IF on frozen rat brain tissue by Paraformaldehyde perfusion ,  but  there is no positive expression.

Asked by: h123
Thank you for your question. This mouse monoclonal, AQP4 (4/18): sc-32739, is suitable for that research application. We recommend using the primary antibody at a starting dilution of 1:50 (range of 1:50-1:500). And since you are using Paraformaldehyde, we recommend including a cell permeabilization treatment (e.g., Triton X-100). A link to our recommended protocol is below. This antibody has also been cited in the literature for IF on rat brain tissue. As one example, please refer to the following publication on NCBI (PMID: 22420318). https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining Please contact our Technical Support Department for additional support. Please call our Asian Support office toll-free at 800-820-8626, email asia@scbio.cn, or contact us by live chat.
Answered by: Technical Support
Date published: 2017-02-14

Why does your Western blot data show doublet bands with AQP4 (4/18): sc-32739 monoclonal antibody?

Asked by: jenni
AQP4 consists of several phosphorylation and glycosylation sites, therefore, the doublet bands that appear on our Western blot may be due to post-translational modifications of AQP4.
Answered by: Technical Support 15
Date published: 2019-01-28

Can AQP4 (4/18): sc-32739 this antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Thank you for your question. Yes, AQP4 (4/18): sc-32739 monoclonal antibody has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 15
Date published: 2017-01-12
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Rated 5 out of 5 by from Ottimo anticorpoHo recentemente provato questo anticorpo in IF, su fettine di midollo spinale di topo criostatate. Diluizione 1:200 e funziona bene, il segnale è pulito e specifico.Ottimo prodotto. Funziona molto bene anche per co-immuno
Date published: 2019-09-18
Rated 1 out of 5 by from 小鼠脑片AQP4一直染不出来我按照1:50的浓度染,没有;按照1:25染还是没有。用枸橼酸钠修复了,triton通透了,但还是没有
Date published: 2018-08-11
Rated 4 out of 5 by from work well in WB at 1:200, with hippocampuswork well in WB at 1:200, with hippocampus,but degrade in storage of -20°C
Date published: 2018-05-07
Rated 1 out of 5 by from Not working on Western blotTrial antibody did not work by Western blot on primary rat astrocyte samples. Blank blots only regardless of antibody dilution, or blocking buffer.
Date published: 2018-04-10
Rated 1 out of 5 by from Didn't work in hek293TUsed AQP4 at a 1:200 dilution for WB on NuPAGE gel using HEK293T WCE. Unfortunately, we weren't able to detect anything.
Date published: 2017-09-29
Rated 5 out of 5 by from 研究血脑屏障以前用过,WB挺好的,感觉SANTA的抗体染色一直都非常好,就是不知道为啥好多抗体停产了,太可惜了。
Date published: 2017-07-28
Rated 5 out of 5 by from Nice for flow cytommetryIt worked very well for flow cytommetry. You can see a nice shift after permeabilisation with Saponin.
Date published: 2017-07-06
Rated 3 out of 5 by from no expression of AQP4 in bEND3 cellsI used this antibody sample at 1/500 dilution to detect AQP4 in bEND3 cell lysate and detectd no bands between 35 and 40 kD.
Date published: 2017-06-20
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Aquaporin 4/AQP4 Anticorpo (4/18) is rated 4.0 out of 5 by 21.
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