ZnT-10 CRISPR/Cas9 KO Plasmid (h): sc-403451
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ZnT-10 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ZnT-10 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZnT-10 CRISPR/Cas9 KO Plasmid (h) | sc-403451 | 20 µg | $397.00 |
Overview
SLC30A10 encodes ZnT-10, a member of the SLC30 family of metal transporters that regulates intracellular divalent cation homeostasis with a key role in manganese efflux and compartmentalization. By controlling cellular manganese levels, ZnT-10 influences metalloprotein function, oxidative stress responses, and downstream signaling processes that are sensitive to metal-dependent enzymatic activity. Dysregulation of SLC30A10 is linked to abnormal manganese accumulation and altered metal handling that can perturb hepatic and neurological physiology. As a result, ZnT-10 is studied in pathways connecting metal transport, mitochondrial function, and cellular stress adaptation in relevant human cell models.
ZnT-10 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SLC30A10 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SLC30A10 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SLC30A10 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ZnT-10 protein expression.
This CRISPR knockout system enables efficient generation of SLC30A10-deficient cell models for investigation of ZnT-10 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SLC30A10 exon(s) critical for ZnT-10 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SLC30A10 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ZnT-10 CRISPR/Cas9 KO Plasmid (h) and ZnT-10 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SLC30A10 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ZnT-10 HDR Plasmid (h) and ZnT-10 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SLC30A10 homology arms to support homology-directed repair at defined SLC30A10 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.