ZNF217 Double Nickase Plasmid (h): sc-408784-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ZNF217 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ZNF217 Double Nickase Plasmid (h) and ZNF217 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ZNF217. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZNF217 Double Nickase Plasmid (h) | sc-408784-NIC | 20 µg | $410.00 | |||
ZNF217 Double Nickase Plasmid (h2) | sc-408784-NIC-2 | 20 µg | $410.00 |
ZNF217 encodes a nuclear C2H2 zinc-finger transcription factor that functions as a chromatin-associated regulator of gene expression through interactions with corepressor and epigenetic-modifying complexes. It influences transcriptional programs linked to cell-cycle progression, differentiation, and DNA damage responses, and has been connected to altered signaling networks such as PI3K/AKT and TGF-β/SMAD via downstream gene regulation. Dysregulated ZNF217 activity is frequently studied in the context of oncogenic transcriptional reprogramming, aneuploidy, and metastasis-associated phenotypes. As a result, ZNF217 serves as a useful node for dissecting chromatin-state control of gene expression and pathway cross-talk in human cells.
ZNF217 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ZNF217 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ZNF217. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ZNF217 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ZNF217-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.