Date published: 2025-10-27

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ZNF215 Antibody (6L1): sc-135611

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Datasheets
  • ZNF215 Antibody (6L1) is a mouse monoclonal IgG1 κ ZNF215 antibody provided at 100 µg/ml
  • raised against recombinant ZNF215 protein of human origin
  • recommended for detection of ZNF215 of human origin by WB, IP and ELISA
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for ZNF215 Antibody (6L1). This work is in progress.

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    ZNF215 Antibody (6L1) is a mouse monoclonal IgG1 kappa light chain antibody that detects ZNF215 protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-ZNF215 antibody (6L1) is available as the non-conjugated format. ZNF215, also known as BAZ2 or ZKSCAN11, is a crucial member of the Krüppel C2H2-type zinc-finger protein family, characterized by its four C2H2-type zinc fingers, one KRAB domain, and one SCAN box domain, comprising 517 amino acids. ZNF215 plays a significant role in transcriptional regulation, primarily through DNA and protein interactions, which is vital for gene expression modulation. ZNF215 is predominantly expressed in the testis, where ZNF215 is involved in the regulation of genes essential for spermatogenesis. Defects in ZNF215 have been linked to male factor subfertility, highlighting ZNF215′s importance in reproductive health. ZNF215′s ability to recruit histone-modifying proteins via its KRAB domain further underscores ZNF215′s role in chromatin remodeling and gene regulation, making ZNF215 (6L1) monoclonal antibody an essential tool for researchers studying transcriptional mechanisms and fertility-related disorders.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

    LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

    ZNF215 Antibody (6L1) References:

    1. Disruption of a novel imprinted zinc-finger gene, ZNF215, in Beckwith-Wiedemann syndrome.  |  Alders, M., et al. 2000. Am J Hum Genet. 66: 1473-84. PMID: 10762538
    2. Zinc finger proteins: new insights into structural and functional diversity.  |  Laity, JH., et al. 2001. Curr Opin Struct Biol. 11: 39-46. PMID: 11179890
    3. Chromosomal region 11p15 is associated with male factor subfertility.  |  Gianotten, J., et al. 2003. Mol Hum Reprod. 9: 587-92. PMID: 12970396
    4. The SCAN domain family of zinc finger transcription factors.  |  Edelstein, LC. and Collins, T. 2005. Gene. 359: 1-17. PMID: 16139965
    5. Multiple genes encoding zinc finger domains are expressed in human T cells.  |  Thiesen, HJ. 1990. New Biol. 2: 363-74. PMID: 2288909
    6. Development of Castration Resistant Prostate Cancer can be Predicted by a DNA Hypermethylation Profile.  |  Angulo, JC., et al. 2016. J Urol. 195: 619-26. PMID: 26551297
    7. Parent-of-origin tumourigenesis is mediated by an essential imprinted modifier in SDHD-linked paragangliomas: SLC22A18 and CDKN1C are candidate tumour modifiers.  |  Hoekstra, AS., et al. 2016. Hum Mol Genet. 25: 3715-3728. PMID: 27402879
    8. Finger proteins and DNA-specific recognition: distinct patterns of conserved amino acids suggest different evolutionary modes.  |  Payre, F. and Vincent, A. 1988. FEBS Lett. 234: 245-50. PMID: 3292287
    9. Loss of ZNF215 imprinting is associated with poor five-year survival in patients with cytogenetically abnormal-acute myeloid leukemia.  |  Yang, MY., et al. 2021. Blood Cells Mol Dis. 90: 102577. PMID: 34091126
    10. CircZNF215 promotes tumor growth and metastasis through inactivation of the PTEN/AKT pathway in intrahepatic cholangiocarcinoma.  |  Liao, W., et al. 2023. J Exp Clin Cancer Res. 42: 125. PMID: 37198696
    11. Bidirectional Mediation Effects between Intratumoral Microbiome and Host DNA Methylation Changes Contribute to Stomach Adenocarcinoma.  |  Yue, K., et al. 2023. Microbiol Spectr. 11: e0090423. PMID: 37260411
    12. Zinc fingers: conserved properties that can distinguish between spurious and actual DNA-binding motifs.  |  Rosenfeld, R. and Margalit, H. 1993. J Biomol Struct Dyn. 11: 557-70. PMID: 8129873

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    ZNF215 Antibody (6L1)

    sc-135611
    100 µg/ml
    $333.00

    For Western Blot, is it recommended to use denatured or non-denatured conditions with ZNF215 (6L1): sc-135611 antibody?

    Asked by: TinTin
    Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
    Answered by: Technical Support
    Date published: 2017-03-27
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    Rated 3 out of 5 by from Good specificity in WB in human transfectedGood specificity in WB in human transfected 293T whole cell lysates. -SCBT QC
    Date published: 2013-03-11
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    ZNF215 Antibody (6L1) is rated 3.0 out of 5 by 1.
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