ZNF101 CRISPR/Cas9 KO Plasmid (h): sc-413991
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ZNF101 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ZNF101 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZNF101 CRISPR/Cas9 KO Plasmid (h) | sc-413991 | 20 µg | $397.00 |
Overview
ZNF101 encodes a C2H2-type zinc finger protein predicted to function as a sequence-specific DNA-binding regulator of transcription. As part of zinc finger transcription factor networks, ZNF101 is expected to contribute to chromatin-associated control of gene expression programs that influence cell identity, proliferation, and stress responses. Perturbation of zinc finger–mediated transcriptional regulation is frequently linked to dysregulated epigenetic states and oncogenic or developmental phenotypes, making ZNF101 relevant for mechanistic studies of gene regulatory circuitry. Human ZNF101 is therefore of interest in mapping transcriptional control pathways and identifying context-dependent roles in cellular homeostasis and disease-associated gene expression signatures.
ZNF101 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ZNF101 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ZNF101 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ZNF101 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ZNF101 protein expression.
This CRISPR knockout system enables efficient generation of ZNF101-deficient cell models for investigation of ZNF101 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ZNF101 exon(s) critical for ZNF101 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ZNF101 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ZNF101 CRISPR/Cas9 KO Plasmid (h) and ZNF101 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ZNF101 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ZNF101 HDR Plasmid (h) and ZNF101 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ZNF101 homology arms to support homology-directed repair at defined ZNF101 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.