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Z-VAD(OMe)-FMK (CAS 187389-52-2) - chemical structure image
Molecular structure of Z-VAD(OMe)-FMK, CAS Number: 187389-52-2
Z-VAD(OMe)-FMK (CAS 187389-52-2) - chemical structure image
Molecular structure of Z-VAD(OMe)-FMK, CAS Number: 187389-52-2
Molecular structure of Z-VAD(OMe)-FMK, CAS Number: 187389-52-2

Z-VAD(OMe)-FMK (CAS 187389-52-2)

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Alternate Names:
Z-VAD(OMe)-FMK also known as Z-VAD-FMK; pan-Caspase Inhibitor; Caspase family inhibitor (fluoromethylketone); Z-Val-Ala-Asp(OMe)-fluoromethylketone; Z-VAD(OMe)-fluoromethylketone, Caspase Inhibitor (FMK); Z-Val-Ala-Asp fluoromethyl ketone
Application:
Z-VAD(OMe)-FMK is an irreversible and cell permeable broad-spectrum Caspase Inhibitor that has shown to inhibit caspase-1 and caspase-3
CAS Number:
187389-52-2
Purity:
≥95%
Molecular Weight:
467.49
Molecular Formula:
C22H30FN3O7
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Description
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SDS & Certificate of Analysis

Z-VAD(OMe)-FMK is an irreversible and cell permeable broad-spectrum Caspase Inhibitor displaying an IC50 of 5.8 nM in HepG2 cells. Z-VAD(OMe)-FMK may act to inhibit caspase-1 and caspase-3. Z-VAD(OMe)-FMK also been shown to inhibit Fas-mediated apoptosis in Jurkat T cells and apoptosis in THP.1 cells induced by diverse stimuli (including cycloheximide, thapsigargin, etoposide and staurosporine). Furthermore, anti-CD95-induced hepatocyte apoptosis was prevented in a concentration-dependent manner by inhibitor Z-VAD-FMK (IC50 = 1.5 nM). Additionally, Z-VAD(OMe)-FMK has been shown that pretreatment of MDA-MB-231 and HL-60 cells with Z-VAD(OMe)-FMK also inhibits apoptosis.


Z-VAD(OMe)-FMK (CAS 187389-52-2) References

  1. Monocytic cell necrosis is mediated by potassium depletion and caspase-like proteases.  |  Warny, M. and Kelly, CP. 1999. Am J Physiol. 276: C717-24. PMID: 10070000
  2. TNF-induced enterocyte apoptosis and detachment in mice: induction of caspases and prevention by a caspase inhibitor, ZVAD-fmk.  |  Piguet, PF., et al. 1999. Lab Invest. 79: 495-500. PMID: 10212002
  3. Correlation of tumor necrosis factor alpha (TNF alpha) with high Caspase 3-like activity in myelodysplastic syndromes.  |  Mundle, SD., et al. 1999. Cancer Lett. 140: 201-7. PMID: 10403560
  4. A specific inhibitor of apoptosis decreases tissue injury after intestinal ischemia-reperfusion in mice.  |  Farber, A., et al. 1999. J Vasc Surg. 30: 752-60. PMID: 10514215
  5. Protective effect of a caspase inhibitor in models for cerebral ischemia in vitro and in vivo.  |  Wiessner, C., et al. 2000. Cell Mol Biol (Noisy-le-grand). 46: 53-62. PMID: 10726971
  6. Serine protease inhibitors suppress cytochrome c-mediatedcaspase-9 activation and apoptosis during hypoxia-reoxygenation.  |  Dong, Z., et al. 2000. Biochem J. 347 Pt 3: 669-77. PMID: 10769169
  7. Computer algorithm for the tomographic image reconstruction with X-ray transmission scans.  |  Cho, ZH. and Ahn, IS. 1975. Comput Biomed Res. 8: 8-25. PMID: 1122749
  8. Differential regulation of maturation and apoptosis of human monocyte-derived dendritic cells mediated by MHC class II.  |  Lokshin, AE., et al. 2002. Int Immunol. 14: 1027-37. PMID: 12202400
  9. Nitric oxide-induced mitochondrial fission is regulated by dynamin-related GTPases in neurons.  |  Barsoum, MJ., et al. 2006. EMBO J. 25: 3900-11. PMID: 16874299
  10. Ru(II)-Thymine Complex Causes Cell Growth Inhibition and Induction of Caspase-Mediated Apoptosis in Human Promyelocytic Leukemia HL-60 Cells.  |  de Souza Oliveira, M., et al. 2018. Int J Mol Sci. 19: PMID: 29848969
  11. ABT-737 ameliorates docetaxel resistance in triple negative breast cancer cell line.  |  Hwang, E., et al. 2018. Ann Surg Treat Res. 95: 240-248. PMID: 30402442
  12. Involvement of multiple proteases during Fas-mediated apoptosis in T lymphocytes.  |  Chow, SC., et al. 1995. FEBS Lett. 364: 134-8. PMID: 7538469
  13. An ICE-like protease is a common mediator of apoptosis induced by diverse stimuli in human monocytic THP.1 cells.  |  Zhu, H., et al. 1995. FEBS Lett. 374: 303-8. PMID: 7589559
  14. Benzyloxycarbonyl-Val-Ala-Asp (OMe) fluoromethylketone (Z-VAD.FMK) inhibits apoptosis by blocking the processing of CPP32.  |  Slee, EA., et al. 1996. Biochem J. 315 (Pt 1): 21-4. PMID: 8670109
  15. Different interleukin-1 beta converting enzyme (ICE) family protease requirements for the apoptotic death of T lymphocytes triggered by diverse stimuli.  |  Sarin, A., et al. 1996. J Exp Med. 184: 2445-50. PMID: 8976202
  16. ICE-protease inhibitors block murine liver injury and apoptosis caused by CD95 or by TNF-alpha.  |  Künstle, G., et al. 1997. Immunol Lett. 55: 5-10. PMID: 9093874
  17. Activation of PKCalpha downstream from caspases during apoptosis induced by 7-hydroxystaurosporine or the topoisomerase inhibitors, camptothecin and etoposide, in human myeloid leukemia HL60 cells.  |  Shao, RG., et al. 1997. J Biol Chem. 272: 31321-5. PMID: 9395460
  18. Different subcellular distribution of caspase-3 and caspase-7 following Fas-induced apoptosis in mouse liver.  |  Chandler, JM., et al. 1998. J Biol Chem. 273: 10815-8. PMID: 9556551

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Z-VAD(OMe)-FMK, 1 mg

sc-311561
1 mg
$135.00

Z-VAD(OMe)-FMK, 5 mg

sc-311561A
5 mg
$530.00

Z-VAD(OMe)-FMK, 10 mg

sc-311561B
10 mg
$1020.00

I need to get 600mg of Z-VAD(OMe)-FMK [sc-311561]. Please provide me with a quote for this purchase at your earliest continent. Also, CHOP is nonprofit hospital. Will be get any discount because of this? Regards

Asked by: CHOP
Thank you for your question. The product is currently in stock. We offer over night shipment to many locations. Please contact our Customer Service department to place a product order or to ask for a sales quotation inlcuding delivery time.
Answered by: Technical Support
Date published: 2018-11-26

What is the half life of this product if it is added into the culture medium?

Asked by: two2igm05
The half-life of this product in vitro is ≤40 minutes.
Answered by: Chemical Support 4
Date published: 2017-02-25

How do I use Z-VAD(OMe)-FMK to inhibit caspase-3/7? 

Asked by: SCM4
The publication by Fox, et al. (PMID: 26771236) describes the use of “z-VAD” to inhibit caspase-3/7.
Answered by: Tech Service 11
Date published: 2017-02-24
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Rated 5 out of 5 by from Excellent pan-caspase inhibitorThis is an excellent pan-caspase inhibitor widely used in the literature.
Date published: 2022-05-29
Rated 5 out of 5 by from Good prduct, good technical serviceI made a purchase of z-VAD(OMe)-FMK to replicate previous findings in other AML cell lines. The current line under investigation undergoes apoptosis in response to a test compound with slower kinetics than other lines with which I had already worked. Still, I was able to show inhibition of apoptosis with 50 and 100 uM z-VAD out to 16 hours with partial effect of 50 uM at 24 hours. It seems that the higher dose is required for later time points in my AML cell lines. (I had previously seen nearly complete reversal of cell death at 10 hours using 50 uM z-VAD in other lines). Additionally, I was able to easily dissolve the compound at 100mM stock solution and it survived two freeze-thaw cycles and retained its activity. Overall, a good product. In addition, when an overnight shipment failed to arrive on a Friday due to weather and sat in a warehouse at RT over the weekend, the technical service worked with me and eventually sent a new vial for me to test. In the end, the original vial showed activity that was only slightly reduced compared to the new vial that did not have any extended time out of the freezer (as powder).
Date published: 2018-02-06
Rated 5 out of 5 by from the advantages of itit is an irreversible broad-spectrum caspase inhibitor of permeable cells, which can block apoptosis, and the effects of it is good ,but which may be harmful to the environment.
Date published: 2017-01-08
Rated 5 out of 5 by from BergerBerger, AK. et al. (PubMed 23674612) used product sc-311561 (Z-VAD(OMe)-FMK), a cell permeable caspase inhibitor, to determine if reovirus induces caspase-independent cell death. -SCBT Publication Review
Date published: 2015-07-10
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Z-VAD(OMe)-FMK is rated 5.0 out of 5 by 4.
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