Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
XRCC2 CRISPR/Cas9 KO Plasmid (h): sc-403531
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- XRCC2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the XRCC2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: XRCC2 Antibody (F-4): sc-365854
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
XRCC2 CRISPR/Cas9 KO Plasmid (h) | sc-403531 | 20 µg | $397.00 |
Overview
XRCC2 encodes a RAD51 paralog that functions in homologous recombination (HR) repair of DNA double-strand breaks, supporting RAD51 filament assembly and stabilization during strand invasion. This protein is integral to maintaining replication fork integrity and resolving DNA damage arising from replication stress, with close functional connections to the Fanconi anemia pathway and broader genome maintenance networks. Disruption of XRCC2 impairs HR capacity, elevates chromosomal instability, and increases sensitivity to DNA crosslinking agents, linking XRCC2 dysfunction to cancer predisposition and related DNA repair deficiency phenotypes. As a core HR factor, XRCC2 is frequently studied in mechanisms of genome stability, mutational processes, and DNA damage response signaling.
XRCC2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the XRCC2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the XRCC2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the XRCC2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish XRCC2 protein expression.
This CRISPR knockout system enables efficient generation of XRCC2-deficient cell models for investigation of XRCC2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting XRCC2 exon(s) critical for XRCC2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple XRCC2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by XRCC2 CRISPR/Cas9 KO Plasmid (h) and XRCC2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the XRCC2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by XRCC2 HDR Plasmid (h) and XRCC2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by XRCC2 homology arms to support homology-directed repair at defined XRCC2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.