XPA Antibody (3H55): sc-73272
- XPA Antibody (3H55) is a mouse monoclonal IgG2a κ, cited in 1 publications, provided at 200 µg/ml
- raised against full length XPA of human origin
- recommended for detection of XPA of mouse, rat and human origin by WB, IP, IF and IHC(P)
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for XPA Antibody (3H55) for WB and IHC(P) applications. These reagents are now offered in bundles with XPA Antibody (3H55) (see ordering information below).
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XPA Antibody (3H55) is a mouse monoclonal IgG2a light chain antibody that detects Xeroderma pigmentosum group A (XPA) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. XPA plays a crucial role in the nucleotide excision repair (NER) pathway, which is essential for repairing DNA damage caused by ultraviolet (UV) light. XPA protein is primarily located in the nucleus, where XPA coordinates the assembly of a preincision complex necessary for the recognition and excision of damaged DNA. XPA′s nuclear localization is directly involved in maintaining genomic stability; without proper localization and function, cells are unable to effectively repair UV-induced DNA lesions, leading to an increased risk of skin cancers in individuals with xeroderma pigmentosum. XPA′s ability to form a homodimer in the absence of DNA and enhanced binding efficiency when dimerically bound underscores the regulatory mechanisms that cells employ to modulate activity based on DNA damage levels. Anti-XPA antibody (3H55) serves as an invaluable tool for researchers studying the molecular mechanisms of DNA repair and the implications of XPA deficiencies in cancer biology.
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XPA Antibody (3H55) References:
- Xpa and Xpa/p53+/- knockout mice: overview of available data. | van Kreijl, CF., et al. 2001. Toxicol Pathol. 29 Suppl: 117-27. PMID: 11695547
- SIRT1 regulates UV-induced DNA repair through deacetylating XPA. | Fan, W. and Luo, J. 2010. Mol Cell. 39: 247-58. PMID: 20670893
- Both XPA and DNA polymerase eta are necessary for the repair of doxorubicin-induced DNA lesions. | Moraes, MC., et al. 2012. Cancer Lett. 314: 108-18. PMID: 21999933
- XPA-mediated regulation of global nucleotide excision repair by ATR Is p53-dependent and occurs primarily in S-phase. | Li, Z., et al. 2011. PLoS One. 6: e28326. PMID: 22174788
- UV-induced nuclear import of XPA is mediated by importin-α4 in an ATR-dependent manner. | Li, Z., et al. 2013. PLoS One. 8: e68297. PMID: 23861882
- High expression of XPA confers poor prognosis for nasopharyngeal carcinoma patients treated with platinum-based chemoradiotherapy. | Fu, X., et al. 2015. Oncotarget. 6: 28478-90. PMID: 26156020
- RPA and XPA interaction with DNA structures mimicking intermediates of the late stages in nucleotide excision repair. | Krasikova, YS., et al. 2018. PLoS One. 13: e0190782. PMID: 29320546
- ERCC1-XPF targeting to psoralen-DNA crosslinks depends on XPA and FANCD2. | Sabatella, M., et al. 2020. Cell Mol Life Sci. 77: 2005-2016. PMID: 31392348
- Homozygous CRISPR/Cas9 Knockout Generated a Novel Functionally Active Exon 1 Skipping XPA Variant in Melanoma Cells. | Banicka, V., et al. 2022. Int J Mol Sci. 23: PMID: 36232946
- A Significant Increasing Risk Association between Cigarette Smoking and XPA and XPC Genes Polymorphisms. | Almushawwah, S., et al. 2023. Genes (Basel). 14: PMID: 37510255
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
XPA Antibody (3H55) | sc-73272 | 200 µg/ml | $316.00 | |||
XPA Antibody (3H55): m-IgG Fc BP-HRP Bundle | sc-539467 | 200 µg Ab; 10 µg BP | $354.00 | |||
XPA Antibody (3H55): m-IgG2a BP-HRP Bundle | sc-546711 | 200 µg Ab; 10 µg BP | $354.00 |