WAPL Antibody (A-7): sc-365189

WAPL Antibody (A-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects WAPL protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). WAPL (A-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. WAPL protein, also known as wings apart-like or WAPAL, is a 1,190 amino acid protein characterized by the presence of one WAPL domain and is expressed in two alternatively spliced isoforms, with one isoform predominantly localized to the nucleus. This nuclear localization enables WAPL to play a significant role in regulating sister-chromatid cohesion during cell division, which is essential for maintaining genomic stability. Aberrations in this process can lead to an increased risk of cancer development and metastasis, highlighting the importance of WAPL in cellular growth and tumorigenesis. The gene encoding WAPL is located on human chromosome 10, a region that harbors over 1,200 genes and is implicated in various genetic disorders, including Charcot-Marie-Tooth disease and multiple endocrine neoplasia type 2.

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WAPL Antibody (A-7) References:

1. Expression of a novel human gene, human wings apart-like (hWAPL), is associated with cervical carcinogenesis and tumor progression.  |  Oikawa, K., et al. 2004. Cancer Res. 64: 3545-9. PMID: 15150110
2. Identification and cloning of a novel chromatin-associated protein partner of Epstein-Barr nuclear protein 2.  |  Kwiatkowski, BA., et al. 2004. Exp Cell Res. 300: 223-33. PMID: 15383329
3. A dioxin sensitive gene, mammalian WAPL, is implicated in spermatogenesis.  |  Kuroda, M., et al. 2005. FEBS Lett. 579: 167-72. PMID: 15620708
4. Human Wapl is a cohesin-binding protein that promotes sister-chromatid resolution in mitotic prophase.  |  Gandhi, R., et al. 2006. Curr Biol. 16: 2406-17. PMID: 17112726
5. Wapl controls the dynamic association of cohesin with chromatin.  |  Kueng, S., et al. 2006. Cell. 127: 955-67. PMID: 17113138
6. Unscheduled overexpression of human WAPL promotes chromosomal instability.  |  Ohbayashi, T., et al. 2007. Biochem Biophys Res Commun. 356: 699-704. PMID: 17382297
7. Eco1-dependent cohesin acetylation during establishment of sister chromatid cohesion.  |  Rolef Ben-Shahar, T., et al. 2008. Science. 321: 563-6. PMID: 18653893
8. Expression of various types of alternatively spliced WAPL transcripts in human cervical epithelia.  |  Oikawa, K., et al. 2008. Gene. 423: 57-62. PMID: 18662753
9. The Cohesin Release Factor WAPL Restricts Chromatin Loop Extension.  |  Haarhuis, JHI., et al. 2017. Cell. 169: 693-707.e14. PMID: 28475897
10. Topologically associating domains and chromatin loops depend on cohesin and are regulated by CTCF, WAPL, and PDS5 proteins.  |  Wutz, G., et al. 2017. EMBO J. 36: 3573-3599. PMID: 29217591
11. WAPL induces cervical intraepithelial neoplasia modulated with estrogen signaling without HPV E6/E7.  |  Kumagai, K., et al. 2021. Oncogene. 40: 3695-3706. PMID: 33947962
12. Enhancer-promoter interactions and transcription are largely maintained upon acute loss of CTCF, cohesin, WAPL or YY1.  |  Hsieh, TS., et al. 2022. Nat Genet. 54: 1919-1932. PMID: 36471071

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-WAPL Antibody (A-7) has 15 citations in a variety of scientific publications.

Primary antibodies like Anti-WAPL Antibody (A-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

WAPL Antibody (A-7) Specifications

Alternate Product Name: WAPL

Product Clone Name: A-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-300

Epitope Target: WAPL

Epitope Species: human

Product Reactivity/Detection: WAPL

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 15 citations in various scientific publications

Available Conjugates/Forms for WAPL Antibody (A-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin