Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
utrophin CRISPR/Cas9 KO Plasmid (h2): sc-400522-KO-2
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- utrophin CRISPR/Cas9 Knockout (KO) Plasmid (h2) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the utrophin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: utrophin Antibody (8A4): sc-33700
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
utrophin CRISPR/Cas9 KO Plasmid (h2) | sc-400522-KO-2 | 20 µg | $397.00 |
Overview
UTRN encodes utrophin, a large cytoskeletal linker protein that connects the actin cytoskeleton to the dystrophin-associated glycoprotein complex at the sarcolemma and other membrane domains. Utrophin contributes to membrane stability, mechanotransduction, and organization of cell–matrix adhesion structures, supporting signaling and structural integrity in striated muscle and additional tissues. Through its spectrin-like repeats and interaction interfaces, utrophin helps coordinate cytoskeletal remodeling and membrane-associated scaffolding processes. Altered UTRN regulation and compensatory utrophin expression are widely studied in the context of muscular dystrophy biology and related membrane fragility phenotypes.
utrophin CRISPR/Cas9 KO Plasmid (h2) is a pool of plasmids designed for targeted disruption of the UTRN gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the UTRN together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the UTRN open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish utrophin protein expression.
This CRISPR knockout system enables efficient generation of UTRN-deficient cell models for investigation of utrophin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting UTRN exon(s) critical for utrophin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple UTRN genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by utrophin CRISPR/Cas9 KO Plasmid (h) and utrophin CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the UTRN locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by utrophin HDR Plasmid (h) and utrophin HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by UTRN homology arms to support homology-directed repair at defined UTRN target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.