UNC5H1 CRISPR/Cas9 KO Plasmid (h): sc-405369
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- UNC5H1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the UNC5H1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UNC5H1 CRISPR/Cas9 KO Plasmid (h) | sc-405369 | 20 µg | $397.00 |
Overview
UNC5A encodes the netrin receptor UNC5H1, a transmembrane guidance molecule that directs axon navigation and neuronal positioning through ligand-dependent signaling. UNC5H1 participates in netrin-1–mediated attractive/repulsive cue integration and can influence cytoskeletal dynamics, adhesion, and migratory behavior via downstream Rho family GTPase and MAPK-linked pathways. Beyond neurodevelopment, UNC5H1-associated signaling has been connected to regulation of apoptosis and epithelial–mesenchymal-like cellular programs, supporting roles in tissue patterning and homeostasis. Dysregulated UNC5A/UNC5H1 expression or altered pathway coupling has been reported in studies of neurodevelopmental disorders and cancer biology, where it is used as a mechanistic node to probe guidance-to-survival cross-talk.
UNC5H1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the UNC5A gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the UNC5A together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the UNC5A open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish UNC5H1 protein expression.
This CRISPR knockout system enables efficient generation of UNC5A-deficient cell models for investigation of UNC5H1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting UNC5A exon(s) critical for UNC5H1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple UNC5A genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by UNC5H1 CRISPR/Cas9 KO Plasmid (h) and UNC5H1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the UNC5A locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by UNC5H1 HDR Plasmid (h) and UNC5H1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by UNC5A homology arms to support homology-directed repair at defined UNC5A target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.