UltraCruz^® Hard-set Mounting Medium with DAPI: sc-359850

UltraCruz® Hard-set Mounting Medium with DAPI is a specialized chemical compound primarily used in microscopy and cellular biology to preserve and visualize DNA within cell preparations. This mounting medium contains DAPI (4′,6-diamidino-2-phenylindole), a fluorescent stain that binds strongly to the adenine-thymine rich regions in DNA, emitting a bright blue fluorescence when exposed to ultraviolet light. This specific binding characteristic makes DAPI an invaluable tool for identifying and quantifying nuclei and chromosomes in cell preparations. The medium itself is designed to harden after application, creating a permanent seal that preserves the integrity and morphology of the specimen on the microscope slide. This hard-setting property prevents the displacement or loss of the sample, which is essential for long-term storage and detailed analysis. Researchers utilize UltraCruz® Hard-set Mounting Medium with DAPI extensively in fields such as genetics, cell biology, and histology to study cell structures, conduct cell counting, and perform localization studies within tissues. The combination of its fluorescence capabilities and preservation qualities makes it ideal for both routine laboratory examinations and complex research projects that require precise cellular and subcellular visualizations.

UltraCruz^® Hard-set Mounting Medium with DAPI References:

1. Long-term alterations of colonic nerve-mast cell interactions induced by neonatal maternal deprivation in rats.  |  Barreau, F., et al. 2008. Gut. 57: 582-90. PMID: 18194988
2. Delivery of siRNA into the cytoplasm by liposomal bubbles and ultrasound.  |  Negishi, Y., et al. 2008. J Control Release. 132: 124-30. PMID: 18804499
3. Genetic suppression of ghrelin receptors activates brown adipocyte function and decreases fat storage in rats.  |  Mano-Otagiri, A., et al. 2010. Regul Pept. 160: 81-90. PMID: 19931319
4. Peripheral nerve grafts after cervical spinal cord injury in adult cats.  |  Côtε, MP., et al. 2010. Exp Neurol. 225: 173-82. PMID: 20599980
5. Transretinal degeneration in ageing human retina: a multiphoton microscopy analysis.  |  Lei, Y., et al. 2011. Br J Ophthalmol. 95: 727-30. PMID: 21183516
6. Expression of mitochondrial branched-chain aminotransferase and α-keto-acid dehydrogenase in rat brain: implications for neurotransmitter metabolism.  |  Cole, JT., et al. 2012. Front Neuroanat. 6: 18. PMID: 22654736
7. Systematic mutagenesis of α-synuclein reveals distinct sequence requirements for physiological and pathological activities.  |  Burrε, J., et al. 2012. J Neurosci. 32: 15227-42. PMID: 23100443
8. Divergent Modulation of Nociception by Glutamatergic and GABAergic Neuronal Subpopulations in the Periaqueductal Gray.  |  Samineni, VK., et al. 2017. eNeuro. 4: PMID: 28374016
9. Cre-expressing neurons in visual cortex of Ntsr1-Cre GN220 mice are corticothalamic and are depolarized by acetylcholine.  |  Sundberg, SC., et al. 2018. J Comp Neurol. 526: 120-132. PMID: 28884467
10. Cre-expressing neurons in the cortical white matter of Ntsr1-Cre GN220 mice.  |  Sundberg, SC. and Granseth, B. 2018. Neurosci Lett. 675: 36-40. PMID: 29580883
11. Time course and magnitude of alpha-synuclein inclusion formation and nigrostriatal degeneration in the rat model of synucleinopathy triggered by intrastriatal α-synuclein preformed fibrils.  |  Patterson, JR., et al. 2019. Neurobiol Dis. 130: 104525. PMID: 31276792
12. Role of Projections between Piriform Cortex and Orbitofrontal Cortex in Relapse to Fentanyl Seeking after Palatable Food Choice-Induced Voluntary Abstinence.  |  Reiner, DJ., et al. 2020. J Neurosci. 40: 2485-2497. PMID: 32051327
13. The Impact of Milk Fat Globule Membrane with Exercise on Age-Related Degeneration of Neuromuscular Junctions.  |  Sugita, S., et al. 2021. Nutrients. 13: PMID: 34371820