UBE2J1 Double Nickase Plasmid (m): sc-425050-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- UBE2J1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- UBE2J1 Double Nickase Plasmid (m) and UBE2J1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Ube2j1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: UBE2J1 Antibody (B-6): sc-377002
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UBE2J1 Double Nickase Plasmid (m) | sc-425050-NIC | 20 µg | $410.00 |
Mouse Ube2j1 encodes UBE2J1, an ER membrane–anchored E2 ubiquitin-conjugating enzyme that collaborates with ER-resident E3 ligases to drive ER-associated degradation (ERAD) of misfolded or surplus proteins. By supplying ubiquitin to substrates retrotranslocated from the endoplasmic reticulum, UBE2J1 helps maintain proteostasis and supports adaptive signaling during ER stress, including crosstalk with unfolded protein response pathways. Disruption of ERAD components can alter secretory pathway homeostasis, inflammatory signaling, and sensitivity to proteotoxic stress, making UBE2J1 a relevant node for studying mechanisms that contribute to neurodegeneration, metabolic dysfunction, and cancer-associated proteostasis remodeling. Its activity is also pertinent to investigations of membrane protein quality control and antigen processing linked to ubiquitin-dependent turnover.
UBE2J1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Ube2j1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Ube2j1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Ube2j1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Ube2j1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.