UBE1L2 Double Nickase Plasmid (h): sc-412489-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- UBE1L2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- UBE1L2 Double Nickase Plasmid (h) and UBE1L2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting UBA6. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UBE1L2 Double Nickase Plasmid (h) | sc-412489-NIC | 20 µg | $410.00 | |||
UBE1L2 Double Nickase Plasmid (h2) | sc-412489-NIC-2 | 20 µg | $410.00 |
UBA6 encodes the E1 ubiquitin-activating enzyme UBE1L2, which initiates ubiquitin and ubiquitin-like conjugation by adenylating ubiquitin and transferring it to E2 enzymes to support downstream E3 ligase–mediated substrate modification. This activity helps coordinate ubiquitin-dependent proteostasis, protein quality control, and regulated turnover of signaling factors, thereby influencing cell-cycle progression, stress responses, and innate immune signaling. UBA6 is also linked to the FAT10/UBD pathway through activation of ubiquitin-like modifiers that shape antigen processing and inflammatory signaling. Dysregulation of ubiquitin activation and conjugation cascades is broadly relevant to mechanisms underlying neurodegeneration, cancer-associated signaling rewiring, and inflammatory disease biology, making UBA6 a useful target for dissecting ubiquitin-pathway dependencies.
UBE1L2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the UBA6 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within UBA6. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt UBA6 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of UBA6-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.