TTF-1/Thyroid Transcription Factor 1/NKX2-1 Double Nickase Plasmid (h): sc-400458-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TTF-1/Thyroid Transcription Factor 1/NKX2-1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TTF-1/Thyroid Transcription Factor 1/NKX2-1 Double Nickase Plasmid (h) and TTF-1/Thyroid Transcription Factor 1/NKX2-1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NKX2-1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TTF-1/Thyroid Transcription Factor 1/NKX2-1 Antibody (8G7G3/1): sc-53136
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TTF-1/Thyroid Transcription Factor 1/NKX2-1 Double Nickase Plasmid (h) | sc-400458-NIC | 20 µg | $410.00 | |||
TTF-1/Thyroid Transcription Factor 1/NKX2-1 Double Nickase Plasmid (h2) | sc-400458-NIC-2 | 20 µg | $410.00 |
NKX2-1 encodes TTF-1 (thyroid transcription factor 1), a homeobox DNA-binding transcription factor that directs lineage specification and differentiation programs in thyroid, lung, and ventral forebrain. TTF-1 regulates epithelial identity and organogenesis by coordinating transcriptional networks controlling morphogenesis, hormone biosynthesis pathways in thyroid tissue, and pulmonary surfactant gene expression in airway and alveolar epithelium. Through interactions with cofactors and chromatin regulatory complexes, NKX2-1 integrates developmental signaling inputs to maintain tissue-specific gene expression states. Dysregulation of NKX2-1 is implicated in congenital developmental disorders and is frequently studied in cancer biology as a lineage-associated regulator in thyroid and lung tumors.
TTF-1/Thyroid Transcription Factor 1/NKX2-1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NKX2-1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NKX2-1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NKX2-1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NKX2-1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.