Transcobalamin II Antibody (A-5) recommended for detection of precursor and mature Transcobalamin II of human origin by WB, IP, IF, IHC(P) and ELISA

Transcobalamin II Antibody (A-5): sc-137017

Transcobalamin II Antibody (A-5) is rated 4.2 out of 5 by 5.
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Datasheets

    • Anti-Transcobalamin II Antibody (A-5) is a mouse monoclonal IgG2b (lambda light chain) Transcobalamin II antibody provided at 200 µg/ml
    • raised against amino acids 168-427 mapping at the C-terminus of Transcobalamin II of human origin
    • Transcobalamin II Antibody (A-5) is recommended for detection of precursor and mature Transcobalamin II of human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-Transcobalamin II Antibody (A-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Transcobalamin II (A-5): sc-137017.
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I am using Transcobalamin II (A-5): sc-137017 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jenni
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from IHC for triple negative breast cancer I got a sample for testing this antibody for IHC. The pattern seems cytoplasmic and membrane.
Date published: 2017-10-26
Rated 3 out of 5 by from Get a barely satisfactory result after attempt. I applied the sample 3 months ago, and I used it in western blot in a ratio 1:1000. I got the better result after several times.
Date published: 2017-08-10
Rated 5 out of 5 by from Very Versatile Antibody This antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC and ELISA. Strong, clear signal with little to no background. Beautiful tissue staining.
Date published: 2017-02-03
Rated 5 out of 5 by from Exceptional immunoperoxidase cytoplasmic Exceptional immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human bronchus tissue. -SCBT QC
Date published: 2015-01-08
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