Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TPX2 Antibody (B-10): sc-390183. Western blot analysis of TPX2 expression in untreated HCT-116 (A), chemically-treated HCT-116 (B) and HeLa (C) whole cell lysates. Detection reagent used: m-IgG2b BP-HRP: sc-542741. β-Actin (C4): sc-47778 used as loading control. Detection reagent used: m-IgG Fc BP-HRP: sc-525409.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TPX2 CRISPR/Cas9 KO Plasmid (h): sc-402581
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TPX2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TPX2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TPX2 Antibody (E-2): sc-271570
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TPX2 CRISPR/Cas9 KO Plasmid (h) | sc-402581 | 20 µg | $397.00 |