TNP1 CRISPR/Cas9 KO Plasmid (m): sc-423463
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TNP1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TNP1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TNP1 CRISPR/Cas9 KO Plasmid (m) | sc-423463 | 20 µg | $397.00 |
Overview
Tnp1 encodes transition protein 1 (TNP1), a basic nuclear protein expressed during late spermatogenesis that replaces histones in elongating spermatids prior to protamine deposition. TNP1 promotes chromatin condensation and genome packaging during spermiogenesis, supporting proper nuclear shaping and sperm maturation. Disruption of Tnp1 perturbs the histone-to-protamine transition and can lead to abnormal sperm chromatin structure and reduced male fertility phenotypes in mouse models. As a germ cell–restricted chromatin regulator, TNP1 is widely studied in pathways governing epigenetic reprogramming, DNA compaction, and reproductive biology.
TNP1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Tnp1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Tnp1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Tnp1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TNP1 protein expression.
This CRISPR knockout system enables efficient generation of Tnp1-deficient cell models for investigation of TNP1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Tnp1 exon(s) critical for TNP1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Tnp1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TNP1 CRISPR/Cas9 KO Plasmid (m) and TNP1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Tnp1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TNP1 HDR Plasmid (m) and TNP1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Tnp1 homology arms to support homology-directed repair at defined Tnp1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.