TNF-R2 Antibody (TR75-89) recommended for detection of TNF-R2 of mouse, rat and human origin by WB, IP, IF and FCM

TNF-R2 Antibody (TR75-89): sc-12750

TNF-R2 Antibody (TR75-89) is rated 4.7 out of 5 by 3.
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Datasheets

    • TNF-R2 Antibody (TR75-89) is a Armenian hamster monoclonal IgG provided at 200 µg/ml
    • raised against a purified extracellular domain of TNF-R2 of mouse origin
    • Anti-TNF-R2 Antibody (TR75-89) is recommended for detection of TNF-R2 of mouse, rat and human origin by WB, IP, IF and FCM
    • Anti-TNF-R2 Antibody (TR75-89) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of TNF-R2 (TR75-89): sc-12750.
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Can TNF-R2 (TR75-89): sc-12750 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: TinTin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, TNF-R2 (TR75-89): sc-12750 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Conjugated form is excellent The PE conjugated version of this TNF-R2 antibody performs very well in Flow Cytometry.
Date published: 2017-02-06
Rated 5 out of 5 by from Published good quality flow cytometry data Published good quality flow cytometry data in human ovarian carcinoma cells. -SCBT Publication Review
Date published: 2015-02-01
Rated 4 out of 5 by from Robust FCM analysis of mouse peripheral blood Robust FCM analysis of mouse peripheral blood leukocytes. -SCBT QC
Date published: 2013-02-28
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