TGase1 Antibody (A-5): sc-365821
- TGase1 Antibody (A-5) is a mouse monoclonal IgG2b κ, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 754-787 near the C-terminus of TGase1 of human origin
- Anti-TGase1 Antibody (A-5) is recommended for detection of TGase1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-TGase1 Antibody (A-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP is the preferred secondary detection reagent for TGase1 Antibody (A-5) for WB and IHC(P) applications. This reagent is now offered in a bundle with TGase1 Antibody (A-5) (see ordering information below).
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TGase1 Antibody (A-5) is a mouse monoclonal IgG2b kappa light chain antibody that detects TGase1 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-TGase1 antibody (A-5) is available in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. TGase1 plays a crucial role in forming the cross-linked cell envelope (CE), a vital structure in terminally differentiating mammalian epidermal cells. This 10 to 20 nm thick protein deposit forms on the intracellular surface of the plasma membrane and maintains the integrity and barrier function of the epidermis. The CE is generated through transglutaminases, which catalyze the formation of disulfide bonds and γ-glutamyl-lysine isodipeptide bonds, cross-linking glutamine and lysine residues in substrate proteins. TGase1 influences epidermal differentiation, blood coagulation, and apoptosis through these biochemical processes. Human keratinocyte TGase1 is a membrane-associated protein consisting of 817 amino acids, while human tissue TGase2 is an endothelial cell-specific protein with 687 amino acids, highlighting the diverse roles of transglutaminases in cellular functions and tissue homeostasis.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
TGase1 Antibody (A-5) References:
- Involucrin cross-linking by transglutaminase 1. Binding to membranes directs residue specificity. | Nemes, Z., et al. 1999. J Biol Chem. 274: 11013-21. PMID: 10196183
- Expression of transglutaminase K in normal cervix tissue and cervix carcinomas. | Friedrich, M., et al. 1999. Histochem J. 31: 13-8. PMID: 10405818
- Transglutaminase-1 protects renal epithelial cells from hydrogen peroxide-induced apoptosis through activation of STAT3 and AKT signaling pathways. | Ponnusamy, M., et al. 2009. Am J Physiol Renal Physiol. 297: F1361-70. PMID: 19710241
- Increased transcription of transglutaminase 1 mediates neuronal death in in vitro models of neuronal stress and Aβ1-42-mediated toxicity. | Tripathy, D., et al. 2020. Neurobiol Dis. 140: 104849. PMID: 32222473
- Protein-bound ceramide levels in the epidermis of transglutaminase 1-deficient mice. | Sassa, T., et al. 2021. J Dermatol. 48: 1799-1801. PMID: 34405428
- Cornification of keratinocytes is associated with differential changes in the catalytic activity and the immunoreactivity of transglutaminase-1. | Surbek, M., et al. 2023. Sci Rep. 13: 21550. PMID: 38057394
- Substrate profiling of human transglutaminase 1 using cDNA display and next-generation sequencing. | Munaweera, TIK., et al. 2024. Biosci Biotechnol Biochem. 88: 620-629. PMID: 38479783
- The transglutaminase 1 enzyme is variably acylated by myristate and palmitate during differentiation in epidermal keratinocytes. | Steinert, PM., et al. 1996. J Biol Chem. 271: 26242-50. PMID: 8824274
- Defective stratum corneum and early neonatal death in mice lacking the gene for transglutaminase 1 (keratinocyte transglutaminase). | Matsuki, M., et al. 1998. Proc Natl Acad Sci U S A. 95: 1044-9. PMID: 9448282
- Expression of transglutaminase 1 in human hair follicles, sebaceous glands and sweat glands. | Yoneda, K., et al. 1998. Br J Dermatol. 138: 37-44. PMID: 9536221
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TGase1 Antibody (A-5) | sc-365821 | 200 µg/ml | $322.00 | |||
TGase1 Antibody (A-5): m-IgG2b BP-HRP Bundle | sc-548728 | 200 µg Ab; 10 µg BP | $361.00 | |||
TGase1 Antibody (A-5) AC | sc-365821 AC | 500 µg/ml, 25% agarose | $424.00 | |||
TGase1 Antibody (A-5) HRP | sc-365821 HRP | 200 µg/ml | $322.00 | |||
TGase1 Antibody (A-5) FITC | sc-365821 FITC | 200 µg/ml | $336.00 | |||
TGase1 Antibody (A-5) PE | sc-365821 PE | 200 µg/ml | $349.00 | |||
TGase1 Antibody (A-5) Alexa Fluor® 488 | sc-365821 AF488 | 200 µg/ml | $364.00 | |||
TGase1 Antibody (A-5) Alexa Fluor® 546 | sc-365821 AF546 | 200 µg/ml | $364.00 | |||
TGase1 Antibody (A-5) Alexa Fluor® 594 | sc-365821 AF594 | 200 µg/ml | $364.00 | |||
TGase1 Antibody (A-5) Alexa Fluor® 647 | sc-365821 AF647 | 200 µg/ml | $364.00 | |||
TGase1 Antibody (A-5) Alexa Fluor® 680 | sc-365821 AF680 | 200 µg/ml | $364.00 | |||
TGase1 Antibody (A-5) Alexa Fluor® 790 | sc-365821 AF790 | 200 µg/ml | $364.00 | |||
TGase1 (A-5) Neutralizing Peptide | sc-365821 P | 100 µg/0.5 ml | $69.00 |