TETRAN CRISPR/Cas9 KO Plasmid (h): sc-411436
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TETRAN CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TETRAN genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TETRAN CRISPR/Cas9 KO Plasmid (h) | sc-411436 | 20 µg | $397.00 |
Overview
MFSD10 encodes TETRAN, a multi-pass membrane protein thought to participate in solute transport-related processes and membrane homeostasis, consistent with membership in the major facilitator superfamily. As a putative transporter-like factor, TETRAN is of interest for studies of endomembrane trafficking, organelle function, and cellular metabolic adaptation, where changes in small-molecule flux can modulate stress signaling and downstream transcriptional programs. Altered MFSD10 expression has been reported in transcriptomic datasets across multiple disease contexts, supporting investigation of its contribution to dysregulated metabolism and cell-state transitions. Defining TETRAN function can help clarify how membrane transport networks influence proliferation, survival, and differentiation phenotypes in human cells.
TETRAN CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MFSD10 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MFSD10 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MFSD10 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TETRAN protein expression.
This CRISPR knockout system enables efficient generation of MFSD10-deficient cell models for investigation of TETRAN signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MFSD10 exon(s) critical for TETRAN function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MFSD10 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TETRAN CRISPR/Cas9 KO Plasmid (h) and TETRAN CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MFSD10 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TETRAN HDR Plasmid (h) and TETRAN HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MFSD10 homology arms to support homology-directed repair at defined MFSD10 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.