TEF-4 Antibody (C-10): sc-518181

TEF-4 Antibody (C-10) is a mouse monoclonal IgM antibody that detects TEF-4 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TEF-4 (C-10) antibody is available in a non-conjugated form, allowing for versatile experimental use. The transcriptional enhancer factor (TEF)/TEAD family, which includes TEF-1, TEF-3, TEF-4, and TEF-5, is characterized by a highly conserved 68-amino acid TEA/ATTS DNA-binding domain that interacts with specific enhancers, including SV40 GT-IIC and muscle-specific M-CAT. TEF-4 plays a crucial role in cellular processes, being strongly coexpressed with TEF-1 in mouse mitotic neuroblasts and also found in the gut and nephrogenic regions of the kidney. TEF-4 is essential for mediating mitogenic signals through association with the transcriptional coactivator YAP65, which enhances cellular proliferation. Furthermore, TEF-4 is involved in the regulation of phosphatidylcholine biosynthesis by promoting the activation of CTP:phosphocholine cytidylyltransferase (CCT) alpha, the rate-limiting enzyme in this pathway, through the enhancement of Ets-1 transcriptional activity. The ability of TEF-4 to interact with these critical pathways underscores its importance in developmental biology and cellular signaling, making anti-TEF-4 antibody (C-10) a valuable tool for researchers studying these processes.

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TEF-4 Antibody (C-10) References:

1. Identification of Ets-1 as an important transcriptional activator of CTP:phosphocholine cytidylyltransferase alpha in COS-7 cells and co-activation with transcriptional enhancer factor-4.  |  Sugimoto, H., et al. 2003. J Biol Chem. 278: 19716-22. PMID: 12642588
2. Redundant roles of Tead1 and Tead2 in notochord development and the regulation of cell proliferation and survival.  |  Sawada, A., et al. 2008. Mol Cell Biol. 28: 3177-89. PMID: 18332127
3. Structural and functional analysis of the YAP-binding domain of human TEAD2.  |  Tian, W., et al. 2010. Proc Natl Acad Sci U S A. 107: 7293-8. PMID: 20368466
4. Regulation of mouse embryonic stem cell self-renewal by a Yes-YAP-TEAD2 signaling pathway downstream of LIF.  |  Tamm, C., et al. 2011. J Cell Sci. 124: 1136-44. PMID: 21385842
5. TEAD2, a Hippo pathway gene, is somatically mutated in gastric and colorectal cancers with high microsatellite instability.  |  Je, EM., et al. 2015. APMIS. 123: 359-60. PMID: 25557945
6. MicroRNA‑608 sensitizes non‑small cell lung cancer cells to cisplatin by targeting TEAD2.  |  Wang, Y., et al. 2019. Mol Med Rep. 20: 3519-3526. PMID: 31485614
7. TEAD2 as a novel prognostic factor for hepatocellular carcinoma.  |  Joo, JS., et al. 2020. Oncol Rep. 43: 1785-1796. PMID: 32323824
8. PHF5A promotes colorectal cancerprogression by alternative splicing of TEAD2.  |  Chang, Y., et al. 2021. Mol Ther Nucleic Acids. 26: 1215-1227. PMID: 34853721
9. YY1-induced DLEU1/miR-149-5p Promotes Malignant Biological Behavior of Cholangiocarcinoma through Upregulating YAP1/TEAD2/SOX2.  |  Li, J., et al. 2022. Int J Biol Sci. 18: 4301-4315. PMID: 35864972
10. Localization of human transcription factor TEF-4 and TEF-5 (TEAD2, TEAD3) genes to chromosomes 19q13.3 and 6p21.2 using fluorescence in situ hybridization and radiation hybrid analysis.  |  Jacquemin, P., et al. 1999. Genomics. 55: 127-9. PMID: 9889009

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TEF-4 Antibody (C-10) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-TEF-4 Antibody (C-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TEF-4 Antibody (C-10) Specifications

Alternate Product Name: TEF-4

Product Clone Name: C-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 279-299

Epitope Target: TEF-4

Epitope Species: human

Product Reactivity/Detection: TEF-4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for TEF-4 Antibody (C-10): N/A

The preferred TEF-4 antibody is TEF-4 Antibody (404C5a): sc-81397.