TDRD1 CRISPR/Cas9 KO Plasmid (h): sc-410043
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TDRD1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TDRD1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TDRD1 CRISPR/Cas9 KO Plasmid (h) | sc-410043 | 20 µg | $397.00 |
Overview
TDRD1 (Tudor domain containing 1) is a germline-enriched RNA-binding protein that recognizes symmetrically dimethylated arginine residues on PIWI family proteins and supports assembly of the piRNA pathway. Through these interactions, TDRD1 contributes to transposon silencing, maintenance of genome integrity, and proper progression of spermatogenesis by coordinating piRNA biogenesis and germ granule organization. Disruption of TDRD1-dependent piRNA surveillance is linked to defects in male germ cell development and impaired fertility phenotypes, reflecting its central role in protecting the germline genome. Altered expression and dysregulated germline programs involving TDRD1 have also been investigated in the context of cancer-testis antigen biology and epigenetic deregulation in tumors.
TDRD1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TDRD1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TDRD1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TDRD1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TDRD1 protein expression.
This CRISPR knockout system enables efficient generation of TDRD1-deficient cell models for investigation of TDRD1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TDRD1 exon(s) critical for TDRD1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TDRD1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TDRD1 CRISPR/Cas9 KO Plasmid (h) and TDRD1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TDRD1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TDRD1 HDR Plasmid (h) and TDRD1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TDRD1 homology arms to support homology-directed repair at defined TDRD1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.