TBX3 Antibody (A-6): sc-166623. Near-infrared western blot analysis of TBX3 expression in JAR (A) and U-698-M (B) whole cell lysates. Blocked with UltraCruz® Blocking Reagent: sc-516214. Detection reagent used: m-IgGκ BP-CFL 680: sc-516180.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TBX3 Antibody (A-6): sc-166623. Near-infrared western blot analysis of TBX3 expression in JAR (A) and U-698-M (B) whole cell lysates. Blocked with UltraCruz® Blocking Reagent: sc-516214. Detection reagent used: m-IgGκ BP-CFL 680: sc-516180.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
200 µl of transduction-ready, high-titer CRISPR/dCas9 Lentiviral Activation Particles
TBX3 Lentiviral Activation Particles (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically and efficiently upregulate gene expression via lentiviral transduction of cells
TBX3 Lentiviral Activation Particles (h) contain the following SAM Activation elements: a deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, an MS2-p65-HSF1 fusion protein and a target-specific 20 nt guide RNA. They also contain the blasticidin, hygromycin and puromycin resistance genes
Upon transduction, the SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
gRNAs encoded by TBX3 Lentiviral Activation Plasmid (h) and TBX3 Lentiviral Activation Plasmid (h2) target distinct regulatory regions of the TBX3 promoter. One or both designs may be available
Following transfection, gene activation efficiency can be assayed by WB, IF or IHC using antibody: TBX3 Antibody (A-6): sc-166623