Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TAP CRISPR/Cas9 KO Plasmid (m): sc-424683
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TAP CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TAP genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TAP Antibody (53H8): sc-32319
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TAP CRISPR/Cas9 KO Plasmid (m) | sc-424683 | 20 µg | $397.00 |
Overview
Mouse Nxf1 encodes TAP, a conserved RNA-binding export receptor that partners with NXT1 to drive bulk mRNA transport from the nucleus to the cytoplasm through the nuclear pore complex. TAP recognizes mature messenger ribonucleoprotein particles via interactions with adaptor complexes such as TREX and contributes to coupling of transcription, splicing, and 3′ end processing with nuclear export. By controlling the availability of transcripts for translation, Nxf1 influences proteostasis, cell cycle progression, and stress-responsive gene expression programs. Perturbation of mRNA export machinery is linked to dysregulated gene expression in cancer biology and to neurodevelopmental and neurodegenerative disease mechanisms, making Nxf1 a useful node for studying RNA metabolism–associated pathologies.
TAP CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Nxf1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Nxf1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Nxf1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TAP protein expression.
This CRISPR knockout system enables efficient generation of Nxf1-deficient cell models for investigation of TAP signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Nxf1 exon(s) critical for TAP function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Nxf1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TAP CRISPR/Cas9 KO Plasmid (m) and TAP CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Nxf1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TAP HDR Plasmid (m) and TAP HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Nxf1 homology arms to support homology-directed repair at defined Nxf1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.