T-type Ca++ CP α1G Double Nickase Plasmid (h): sc-418331-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- T-type Ca++ CP α1G Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- T-type Ca++ CP α1G Double Nickase Plasmid (h) and T-type Ca++ CP α1G Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CACNA1G. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
T-type Ca++ CP α1G Double Nickase Plasmid (h) | sc-418331-NIC | 20 µg | $410.00 | |||
T-type Ca++ CP α1G Double Nickase Plasmid (h2) | sc-418331-NIC-2 | 20 µg | $410.00 |
CACNA1G encodes the CaV3.1 (α1G) pore-forming subunit of T-type voltage-gated calcium channels, which activate at low membrane potentials to generate transient Ca2+ influx. This current supports rhythmic firing, rebound burst activity, and calcium-dependent signaling that couples membrane excitability to gene expression programs in excitable and some non-excitable cells. CaV3.1 activity intersects with pathways regulating intracellular Ca2+ homeostasis, neuronal oscillations, and excitation–secretion coupling, influencing processes such as cell-cycle progression and differentiation through calcium-sensitive effectors. Dysregulated CACNA1G expression or channel function has been linked to altered electrical excitability and calcium signaling in neurological and cardiovascular phenotypes, making it a useful target for mechanistic studies of ion channel biology.
T-type Ca++ CP α1G Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CACNA1G locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CACNA1G. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CACNA1G function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CACNA1G-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.