Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SYP/Synaptophysin Double Nickase Plasmid (h): sc-400487-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SYP/Synaptophysin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SYP/Synaptophysin Double Nickase Plasmid (h) and SYP/Synaptophysin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SYP. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SYP/Synaptophysin Antibody (D-4): sc-17750
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SYP/Synaptophysin Double Nickase Plasmid (h) | sc-400487-NIC | 20 µg | $410.00 | |||
SYP/Synaptophysin Double Nickase Plasmid (h2) | sc-400487-NIC-2 | 20 µg | $410.00 |
SYP encodes synaptophysin, a highly abundant integral membrane glycoprotein of synaptic vesicles that supports presynaptic vesicle biogenesis, trafficking, and Ca²⁺-dependent neurotransmitter release. Synaptophysin participates in vesicle recycling and endocytic/exocytic coupling through interactions with vesicle proteins such as synaptobrevin/VAMP2, linking it to synaptic transmission and neuronal network activity. Because SYP is widely used as a marker of synapse density and presynaptic integrity, altered expression or distribution is frequently examined in studies of neurodegeneration, neurodevelopmental disorders, and synaptic dysfunction. Its vesicle-associated localization also makes it relevant for investigations of neuronal differentiation, plasticity, and circuit-level changes driven by disease-associated pathways.
SYP/Synaptophysin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SYP locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SYP. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SYP function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SYP-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.