Synaptopodin Antibody (D-9): sc-515842

Synaptopodin Antibody (D-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects Synaptopodin of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Synaptopodin antibody (D-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Synaptopodin plays a crucial role in the structural integrity and plasticity of dendritic spines, which are essential for synaptic transmission and neuronal communication. Synaptopodin is a proline-rich, actin-associated protein predominantly expressed in mature dendritic spines and renal podocytes, where Synaptopodin links actin filaments to the spine apparatus, facilitating dynamic cytoskeleton remodeling in response to synaptic activity. This remodeling is vital for processes such as long-term potentiation (LTP), as Synaptopodin expression increases during LTP and correlates with its persistence. In the hippocampus, Synaptopodin is localized to the spine neck of principal neurons, while in renal podocytes, Synaptopodin is found in the foot processes, highlighting Synaptopodin′s importance in both neural and renal function. Synaptopodin is absent in the sclerosed glomeruli of idiopathic nephrotic syndrome, indicating Synaptopodin′s potential role as a biomarker for kidney disease. Additionally, Myopodin, a related protein, is expressed in skeletal and cardiac muscle and shares similar actin-bundling properties, suggesting a broader significance of the synaptopodin family in various tissues.

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Synaptopodin Antibody (D-9) References:

1. Actin-associated protein synaptopodin in the rat hippocampal formation: localization in the spine neck and close association with the spine apparatus of principal neurons.  |  Deller, T., et al. 2000. J Comp Neurol. 418: 164-81. PMID: 10701442
2. Potential role of synaptopodin in spine motility by coupling actin to the spine apparatus.  |  Deller, T., et al. 2000. Hippocampus. 10: 569-81. PMID: 11075827
3. Synaptopodin expression in idiopathic nephrotic syndrome of childhood.  |  Srivastava, T., et al. 2001. Kidney Int. 59: 118-25. PMID: 11135064
4. Regulated expression of an actin-associated protein, synaptopodin, during long-term potentiation.  |  Yamazaki, M., et al. 2001. J Neurochem. 79: 192-9. PMID: 11595771
5. Differentiation- and stress-dependent nuclear cytoplasmic redistribution of myopodin, a novel actin-bundling protein.  |  Weins, A., et al. 2001. J Cell Biol. 155: 393-404. PMID: 11673475
6. Myopodin, a synaptopodin homologue, is frequently deleted in invasive prostate cancers.  |  Lin, F., et al. 2001. Am J Pathol. 159: 1603-12. PMID: 11696420
7. Synaptopodin family of natively unfolded, actin binding proteins: physical properties and potential biological functions.  |  Chalovich, JM. and Schroeter, MM. 2010. Biophys Rev. 2: 181-189. PMID: 28510039
8. Synaptopodin-2 Isoforms Have Specific Binding Partners and Display Distinct, Muscle Cell Type-Specific Expression Patterns.  |  Lohanadan, K., et al. 2023. Cells. 13: PMID: 38201288
9. Synaptopodin Regulates Denervation-Induced Plasticity at Hippocampal Mossy Fiber Synapses.  |  Kruse, P., et al. 2024. Cells. 13: PMID: 38247806
10. Loss of synaptopodin impairs mGluR5 and protein synthesis-dependent mGluR-LTD at CA3-CA1 synapses.  |  Wu, PY., et al. 2024. PNAS Nexus. 3: pgae062. PMID: 38384385
11. Synaptopodin is required for long-term depression at Schaffer collateral-CA1 synapses.  |  Inglebert, Y., et al. 2024. Mol Brain. 17: 17. PMID: 38566234
12. Synaptopodin: an actin-associated protein in telencephalic dendrites and renal podocytes.  |  Mundel, P., et al. 1997. J Cell Biol. 139: 193-204. PMID: 9314539

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Synaptopodin Antibody (D-9) has 67 citations in a variety of scientific publications.

Primary antibodies like Anti-Synaptopodin Antibody (D-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Synaptopodin Antibody (D-9) Specifications

Alternate Product Name: Synaptopodin

Product Clone Name: D-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 660-684

Epitope Target: Synaptopodin

Epitope Species: human

Product Reactivity/Detection: Synaptopodin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 67 citations in various scientific publications

Available Conjugates/Forms for Synaptopodin Antibody (D-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin