survivin Antibody (2H5H2) recommended for detection of survivin of mouse, rat and human origin by WB, IP and ELISA

survivin Antibody (2H5H2): sc-101433

survivin Antibody (2H5H2) is rated 3.0 out of 5 by 2.
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Datasheets

    • survivin Antibody (2H5H2) is a mouse monoclonal IgG1 κ, cited in 9 publications, provided at 200 µg/ml
    • raised against full length survivin of human origin
    • recommended for detection of survivin of mouse, rat and human origin by WB, IP and ELISA

How much sample do you recommend loading on the gel for Western blot detection using survivin (2H5H2): sc-101433?

Asked by: Randy McDonald
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 3 out of 5 by from Survivin anti-mouse antibody My experience about this is OK type. I had hard time getting bands which may be because i have low expression of survivin in my cells.
Date published: 2018-02-22
Rated 3 out of 5 by from Clean blot with single band for WB with human Clean blot with single band for WB with human recombinant survivin protein and survivin expression in survivin-GFP transfected COS-7 cells. -SCBT QC
Date published: 2014-03-30
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