Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Stat5b Double Nickase Plasmid (m): sc-423179-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Stat5b Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Stat5b Double Nickase Plasmid (m) and Stat5b Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Stat5b. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Stat5b Antibody (G-2): sc-1656
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Stat5b Double Nickase Plasmid (m) | sc-423179-NIC | 20 µg | $410.00 | |||
Stat5b Double Nickase Plasmid (m2) | sc-423179-NIC-2 | 20 µg | $410.00 |
Mouse Stat5b (signal transducer and activator of transcription 5B) is a cytokine-responsive transcription factor that couples receptor-associated JAK kinases to rapid changes in gene expression. Following phosphorylation, STAT5B dimerizes and translocates to the nucleus to regulate programs controlling lymphocyte development, myeloid differentiation, metabolism, and growth factor signaling, with prominent roles downstream of IL-2 family cytokines and growth hormone. Stat5b activity intersects with JAK–STAT pathway feedback circuits, chromatin remodeling, and transcriptional control of survival and cell-cycle genes. Dysregulated STAT5B signaling is frequently studied in models of immune imbalance, inflammation, and hematopoietic transformation, where altered transcriptional outputs can shift lineage fate and proliferative capacity.
Stat5b Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Stat5b locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Stat5b. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Stat5b function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Stat5b-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.