SPT16 Antibody (A-1): sc-165987
- SPT16 Antibody (A-1) is a mouse monoclonal IgG1 κ, cited in 4 publications, provided at 200 µg/ml
- raised against amino acids 748-1047 mapping at the C-terminus of SPT16 of human origin
- Anti-SPT16 Antibody (A-1) is recommended for detection of SPT16 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-SPT16 Antibody (A-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for SPT16 Antibody (A-1) for WB applications. This reagent is now offered in a bundle with SPT16 Antibody (A-1) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
SPT16 Antibody (A-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects SPT16 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-SPT16 antibody (A-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SPT16 plays a crucial role in transcription by functioning as part of the FACT (facilitates chromatin transcription) complex, which is essential for transcription of chromatin templates. This complex enables nucleosome disassembly during transcription, allowing RNA polymerase to access DNA. SPT16 interacts specifically with nucleosomes and histone H2A/H2B dimers, promoting nucleosome disassembly, which is a key step in transcription elongation. SPT16 forms a complex with structure-specific recognition protein-1 (SSRP-1), which bends DNA and modulates gene expression. The interaction between SPT16 and SSRP-1 enhances transcriptional activity of serum response factors, influencing various gene regulatory pathways. SPT16 serves as a critical component of transcription machinery and a potential target for studies related to gene expression regulation in various biological contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SPT16 Antibody (A-1) References:
- Modulation of SRF-dependent gene expression by association of SPT16 with MKL1. | Kihara, T., et al. 2008. Exp Cell Res. 314: 629-37. PMID: 18036521
- Structure of the human histone chaperone FACT Spt16 N-terminal domain. | Marcianò, G. and Huang, DT. 2016. Acta Crystallogr F Struct Biol Commun. 72: 121-8. PMID: 26841762
- Noncoding Transcription Is a Driving Force for Nucleosome Instability in spt16 Mutant Cells. | Feng, J., et al. 2016. Mol Cell Biol. 36: 1856-67. PMID: 27141053
- The Paramecium histone chaperone Spt16-1 is required for Pgm endonuclease function in programmed genome rearrangements. | de Vanssay, A., et al. 2020. PLoS Genet. 16: e1008949. PMID: 32702045
- Proteasomal Regulation of Mammalian SPT16 in Controlling Transcription. | Kaja, A., et al. 2021. Mol Cell Biol. 41: PMID: 33526453
- SPT16 ubiquitylation by DCAF14-CRL4 regulates FACT binding to histones. | Nakagawa, T., et al. 2022. Cell Rep. 38: 110541. PMID: 35320725
- Phosphorylation of the FACT histone chaperone subunit SPT16 affects chromatin at RNA polymerase II transcriptional start sites in Arabidopsis. | Michl-Holzinger, P., et al. 2022. Nucleic Acids Res. 50: 5014-5028. PMID: 35489065
- Structural basis for H2A-H2B recognitions by human Spt16. | Li, Y. and Huang, H. 2023. Biochem Biophys Res Commun. 651: 85-91. PMID: 36801613
- The histone H2B Arg95 residue efficiently recruits the transcription factor Spt16 to mediate Ste5 expression of the pheromone response pathway. | Sulaiman, AA., et al. 2023. Sci Rep. 13: 10189. PMID: 37349401
- The N-terminus of Spt16 anchors FACT to MCM2-7 for parental histone recycling. | Wang, X., et al. 2023. Nucleic Acids Res. 51: 11549-11567. PMID: 37850662
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SPT16 Antibody (A-1) | sc-165987 | 200 µg/ml | $322.00 | |||
SPT16 Antibody (A-1): m-IgGκ BP-HRP Bundle | sc-521477 | 200 µg Ab, 40 µg BP | $361.00 | |||
SPT16 Antibody (A-1) AC | sc-165987 AC | 500 µg/ml, 25% agarose | $424.00 | |||
SPT16 Antibody (A-1) HRP | sc-165987 HRP | 200 µg/ml | $322.00 | |||
SPT16 Antibody (A-1) FITC | sc-165987 FITC | 200 µg/ml | $336.00 | |||
SPT16 Antibody (A-1) PE | sc-165987 PE | 200 µg/ml | $349.00 | |||
SPT16 Antibody (A-1) Alexa Fluor® 488 | sc-165987 AF488 | 200 µg/ml | $364.00 | |||
SPT16 Antibody (A-1) Alexa Fluor® 546 | sc-165987 AF546 | 200 µg/ml | $364.00 | |||
SPT16 Antibody (A-1) Alexa Fluor® 594 | sc-165987 AF594 | 200 µg/ml | $364.00 | |||
SPT16 Antibody (A-1) Alexa Fluor® 647 | sc-165987 AF647 | 200 µg/ml | $364.00 | |||
SPT16 Antibody (A-1) Alexa Fluor® 680 | sc-165987 AF680 | 200 µg/ml | $364.00 | |||
SPT16 Antibody (A-1) Alexa Fluor® 790 | sc-165987 AF790 | 200 µg/ml | $364.00 |