SPA-1 Antibody (B-3): sc-137047

SPA-1 Antibody (B-3) is a mouse monoclonal IgG1 antibody that detects SPA-1 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SPA-1, or signal-induced proliferation-associated gene-1, serves as a crucial regulator in hematopoietic progenitors and peripheral T cells by acting as a principal GTPase-activating protein for RAP1. SPA-1 is predominantly expressed in fetal and adult lymphohematopoietic tissues, and SPA-1 expression is significantly upregulated in response to various mitogenic stimuli, highlighting SPA-1′s role in immune responses. SPA-1 disrupts the adhesive interactions mediated by LFA-1 and ICAM1, which are essential for T cell receptor triggering and interleukin-2 production, potentially through RAP1 activity inhibition. Studies show that mice lacking the SPA-1 gene exhibit age-dependent T cell immunodeficiency and develop myeloproliferative disorders resembling human chronic myeloid leukemia. SPA-1 interacts directly with aquaporin-2 (AQP2), influencing AQP2 trafficking to the apical membrane, which is vital for maintaining water homeostasis in renal function. Anti-SPA-1 antibody (B-3) serves as an invaluable tool for researchers investigating SPA-1′s complex roles in immune regulation and cellular signaling pathways.

The preferred SPA-1 antibody is SPA-1 Antibody (B-7): sc-166219.