Smurf2 CRISPR/Cas9 KO Plasmid (m): sc-425947
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Smurf2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Smurf2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Smurf2 CRISPR/Cas9 KO Plasmid (m) | sc-425947 | 20 µg | $397.00 |
Overview
Smurf2 (SMAD specific E3 ubiquitin protein ligase 2) is a HECT-type E3 ubiquitin ligase that regulates protein turnover and signal amplitude in multiple pathways, including TGF-β/BMP and Wnt signaling. By ubiquitinating substrates such as receptor-regulated SMADs and associated signaling components, Smurf2 helps control transcriptional programs governing proliferation, differentiation, and epithelial–mesenchymal plasticity. Smurf2 also contributes to genome stability and cellular stress responses through modulation of DNA damage and checkpoint-associated proteins. Dysregulated Smurf2 activity has been linked to altered tissue homeostasis and cancer-relevant phenotypes, including changes in growth control, fibrosis-associated signaling, and metastatic traits, making it a useful node for mechanistic studies in mouse models.
Smurf2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Smurf2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Smurf2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Smurf2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Smurf2 protein expression.
This CRISPR knockout system enables efficient generation of Smurf2-deficient cell models for investigation of Smurf2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Smurf2 exon(s) critical for Smurf2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Smurf2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Smurf2 CRISPR/Cas9 KO Plasmid (m) and Smurf2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Smurf2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Smurf2 HDR Plasmid (m) and Smurf2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Smurf2 homology arms to support homology-directed repair at defined Smurf2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.