smoothelin Antibody (B-1): sc-166292

Smoothelin Antibody (B-1) is a mouse monoclonal IgG2b antibody that detects smoothelin in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Smoothelin is a crucial cytoskeletal protein that plays a significant role in the structure and function of contractile smooth muscle cells, where smoothelin is exclusively expressed. Anti-smoothelin antibody (B-1) associates with actin stress fibers, contributing to the contractile properties of smooth muscle. Notably, smoothelin exists in at least two isoforms produced by alternative splicing, with the short isoform being expressed in visceral muscle tissues like the intestine and stomach, while the long isoform is found in all vascularized organs. Smoothelin expression is particularly important in the vascular system, where smoothelin is limited to large veins and arteries capable of pulsatile contraction, making smoothelin a valuable marker for studying vascular malformations and injuries. The gene encoding human smoothelin is located on chromosome 22q12.2, highlighting smoothelin′s evolutionary conservation among vertebrates and significance in smooth muscle cell differentiation and function. Anti-smoothelin antibody (B-1) is an indispensable tool for researchers investigating the dynamics of smooth muscle cells and their role in various physiological and pathological processes.

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smoothelin Antibody (B-1) References:

1. Diagnostic use of antibody to smoothelin in the recognition of muscularis propria in transurethral resection of urinary bladder tumor (TURBT) specimens.  |  Paner, GP., et al. 2010. Am J Surg Pathol. 34: 792-9. PMID: 20421781
2. Muscularis mucosae versus muscularis propria in gallbladder, cystic duct, and common bile duct: smoothelin and desmin immunohistochemical study.  |  Raparia, K., et al. 2010. Ann Diagn Pathol. 14: 408-12. PMID: 21074688
3. Detection of smoothelin expression in the urinary bladder is strongly dependent on pretreatment conditions: a critical analysis with possible consequences for cancer staging.  |  Lindh, C., et al. 2011. Virchows Arch. 458: 665-70. PMID: 21494761
4. Smoothelin in bladder and gastrointestinal tract again.  |  Aneiros-Fernandez, J., et al. 2011. Histopathology. 58: 1173. PMID: 21707718
5. Smoothelin, a new marker for smooth muscle hamartoma.  |  Espiñeira-Carmona, MJ., et al. 2012. Eur J Dermatol. 22: 549-50. PMID: 22569399
6. A study of α5 chain of collagen IV, caldesmon, placental alkaline phosphatase and smoothelin as immunohistochemical markers of gastrointestinal smooth muscle neoplasms.  |  Wong, NA., et al. 2014. J Clin Pathol. 67: 105-11. PMID: 24043717
7. Smoothelin and caldesmon are reliable markers for distinguishing muscularis propria from desmoplasia: a critical distinction for accurate staging colorectal adenocarcinoma.  |  Roberts, JA., et al. 2014. Int J Clin Exp Pathol. 7: 792-6. PMID: 24551305
8. Pregnancy and Smoothelin-like Protein 1 (SMTNL1) Deletion Promote the Switching of Skeletal Muscle to a Glycolytic Phenotype in Human and Mice.  |  Lontay, B., et al. 2015. J Biol Chem. 290: 17985-17998. PMID: 26048986
9. Value of histomorphometric tumour thickness and smoothelin for conventional m-classification in early oesophageal adenocarcinoma.  |  Endhardt, K., et al. 2017. World J Gastrointest Oncol. 9: 444-451. PMID: 29204253
10. Smoothelin-like protein 1 promotes insulin sensitivity and modulates the contractile properties of endometrial epithelial cells with insulin resistance.  |  Keller, I., et al. 2024. Front Endocrinol (Lausanne). 15: 1375771. PMID: 38883605

The preferred smoothelin antibody is smoothelin Antibody (R4A): sc-23883.