Sm D1 Antibody (A-9): sc-166650

Sm D1 Antibody (A-9) is a mouse monoclonal IgG3 kappa light chain antibody that detects the Sm D1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Sm D1 antibody (A-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Sm D1, also called Small nuclear ribonucleoprotein Sm D1, snRNP core protein D1, and SNRPD1, plays a crucial role in the assembly of small nuclear ribonucleoproteins (snRNPs), which are essential components of the spliceosome, a complex responsible for the splicing of pre-mRNA in eukaryotic cells. By facilitating the binding of snRNPs to the conserved Sm site on U snRNA, Sm D1 helps form a stable snRNP core complex, which is vital for the accurate processing of mRNA. The presence of Sm D1, along with other Sm proteins, ensures the proper functioning of the spliceosome, thereby influencing gene expression and cellular function. Notably, autoantibodies against Sm D1 are commonly found in patients with systemic lupus erythematosus, highlighting Sm D1′s significance in autoimmune responses. The major linear epitope recognized by these autoantibodies includes the C-terminal RG dipeptide repeats present in Sm D1, underscoring Sm D1′s importance in both health and disease.

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Sm D1 Antibody (A-9) References:

1. The C-terminal RG dipeptide repeats of the spliceosomal Sm proteins D1 and D3 contain symmetrical dimethylarginines, which form a major B-cell epitope for anti-Sm autoantibodies.  |  Brahms, H., et al. 2000. J Biol Chem. 275: 17122-9. PMID: 10747894
2. Purified U7 snRNPs lack the Sm proteins D1 and D2 but contain Lsm10, a new 14 kDa Sm D1-like protein.  |  Pillai, RS., et al. 2001. EMBO J. 20: 5470-9. PMID: 11574479
3. Hepatitis C virus nonstructural protein NS3 binds to Sm-D1, a small nuclear ribonucleoprotein associated with autoimmune disease.  |  Iwai, A., et al. 2003. Microbiol Immunol. 47: 601-11. PMID: 14524621
4. Evidence for three distinct D proteins, which react differentially with anti-Sm autoantibodies, in the cores of the major snRNPs U1, U2, U4/U6 and U5.  |  Lehmeier, T., et al. 1990. Nucleic Acids Res. 18: 6475-84. PMID: 1701240
5. SNRPD1 confers diagnostic and therapeutic values on breast cancers through cell cycle regulation.  |  Dai, X., et al. 2021. Cancer Cell Int. 21: 229. PMID: 33879154
6. The diagnostic and prognostic significance of small nuclear ribonucleoprotein Sm D1 aberrantly high expression in hepatocellular carcinoma.  |  Wang, H., et al. 2022. J Cancer. 13: 184-201. PMID: 34976182
7. SNRPD1/E/F/G Serve as Potential Prognostic Biomarkers in Lung Adenocarcinoma.  |  Liu, G., et al. 2022. Front Genet. 13: 813285. PMID: 35356432
8. SNRPD1 conveys prognostic value on breast cancer survival and is required for anthracycline sensitivity.  |  Dai, X., et al. 2023. BMC Cancer. 23: 376. PMID: 37098488
9. SNRPD1 inhibition suppresses the proliferation of hepatocellular carcinoma and promotes autophagy through the PI3K/AKT/mTOR/4EBP1 pathway.  |  Wang, H., et al. 2023. Arch Biochem Biophys. 743: 109661. PMID: 37268273
10. The Potential Role of SNRPD1 Stabilized by IGF2BP2 in the Progression of Triple-Negative Breast Cancer.  |  Liu, S., et al. 2024. Breast Cancer (Dove Med Press). 16: 679-688. PMID: 39411513
11. U2 RNA shares a structural domain with U1, U4, and U5 RNAs.  |  Branlant, C., et al. 1982. EMBO J. 1: 1259-65. PMID: 6202507
12. The snRNP core assembly pathway: identification of stable core protein heteromeric complexes and an snRNP subcore particle in vitro.  |  Raker, VA., et al. 1996. EMBO J. 15: 2256-69. PMID: 8641291

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Sm D1 Antibody (A-9) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-Sm D1 Antibody (A-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Sm D1 Antibody (A-9) Specifications

Alternate Product Name: SNRPD1

Product Clone Name: A-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 84-98

Epitope Target: Sm D1

Epitope Species: human

Product Reactivity/Detection: Sm D1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for Sm D1 Antibody (A-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF647: Alexa Fluor^® 647, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin